Evidence for co-translational misincorporation of non-canonical amino acid hydroxyproline in recombinant antibodies produced in Chinese Hamster Ovary (CHO) cell lines

With the advent of highly sensitive technologies such as tandem mass spectrometry and next-generation sequencing, recombinant antibodies are now routinely analyzed for the presence of low-level sequence variants including amino acid misincorporations. During mAb cell culture process development, we...

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Detalles Bibliográficos
Autores principales: Boddapati, Shanta, Gilmore, Jason, Boone, Kyle, Bushey, John, Ross, Jonathan, Gfeller, Brian, McFee, William, Rao, Romesh, Corrigan, Greg, Chen, Aaron, Clarke, Howard, Valliere-Douglass, John, Bhargava, Swapnil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7595273/
https://www.ncbi.nlm.nih.gov/pubmed/33119652
http://dx.doi.org/10.1371/journal.pone.0241250

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7595273/
https://www.ncbi.nlm.nih.gov/pubmed/33119652
http://dx.doi.org/10.1371/journal.pone.0241250

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