Extensive trimming of short single-stranded DNA oligonucleotides during replication-coupled gene editing in mammalian cells

Through transfection of short single-stranded oligodeoxyribonucleotides (ssODNs) small genomic alterations can be introduced into mammalian cells with high precision. ssODNs integrate into the genome during DNA replication, but the resulting heteroduplex is prone to detection by DNA mismatch repair...

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Detalles Bibliográficos
Autores principales: van Ravesteyn, Thomas W., Arranz Dols, Marcos, Pieters, Wietske, Dekker, Marleen, te Riele, Hein
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7595315/
https://www.ncbi.nlm.nih.gov/pubmed/33119594
http://dx.doi.org/10.1371/journal.pgen.1009041

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7595315/
https://www.ncbi.nlm.nih.gov/pubmed/33119594
http://dx.doi.org/10.1371/journal.pgen.1009041

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