Cargando…
Research Note: A baseline survey of thermotolerant Campylobacter in retail chicken in southern Brazil
Chicken is a leading source of thermotolerant Campylobacter, which triggers human foodborne enteritis. This study evaluated thermotolerant Campylobacter contamination of retail chicken in southern Brazil, using qualitative and quantitative analyses. Selective enrichment in Bolton broth for 24 and 48...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7597543/ https://www.ncbi.nlm.nih.gov/pubmed/32359606 http://dx.doi.org/10.1016/j.psj.2019.12.061 |
_version_ | 1783602384521396224 |
---|---|
author | dos Santos Pozza, Jenifer Voss-Rech, Daiane dos Santos Lopes, Letícia Silveira Luiz Vaz, Clarissa |
author_facet | dos Santos Pozza, Jenifer Voss-Rech, Daiane dos Santos Lopes, Letícia Silveira Luiz Vaz, Clarissa |
author_sort | dos Santos Pozza, Jenifer |
collection | PubMed |
description | Chicken is a leading source of thermotolerant Campylobacter, which triggers human foodborne enteritis. This study evaluated thermotolerant Campylobacter contamination of retail chicken in southern Brazil, using qualitative and quantitative analyses. Selective enrichment in Bolton broth for 24 and 48 h after plating onto modified charcoal–cefoperazone–deoxycholate (mCCD) agar and Preston agar was assessed. The combined results of the detection and enumeration methods revealed a frequency of 70% occurrence of thermotolerant Campylobacter in chicken samples. Campylobacter was enumerated in 60% of the samples, whereas 46% of the samples were positive in the qualitative analysis. Quantitative analysis showed average counts of 3.10 ± 0.15 log(10) CFU/sample. Higher numbers of Campylobacter-positive samples were found using 24-h enrichment before plating onto Preston agar (46%) than onto mCCD agar (2%). The majority of isolated strains were identified as Campylobacter jejuni, and Campylobacter coli was also found but to a lesser extent. Subtyping revealed a clear distinction between strains isolated from different chicken sources. The enriched samples plated onto mCCD agar showed extensive spreading of nonproducing extended-spectrum β-lactamases Proteus mirabilis that hampered the identification of Campylobacter colonies. P. mirabilis strains showed resistance to cefoperazone, trimethoprim, and polymyxin B present in broth and plate media used and were inhibited by rifampicin present in Preston agar. The results underline the effect of the spread of contaminant strains on Campylobacter cultures, which might be prevented using a recently revised International Organization for Standardization method for qualitative analysis of chicken. |
format | Online Article Text |
id | pubmed-7597543 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-75975432020-11-02 Research Note: A baseline survey of thermotolerant Campylobacter in retail chicken in southern Brazil dos Santos Pozza, Jenifer Voss-Rech, Daiane dos Santos Lopes, Letícia Silveira Luiz Vaz, Clarissa Poult Sci Microbiology and Food Safety Chicken is a leading source of thermotolerant Campylobacter, which triggers human foodborne enteritis. This study evaluated thermotolerant Campylobacter contamination of retail chicken in southern Brazil, using qualitative and quantitative analyses. Selective enrichment in Bolton broth for 24 and 48 h after plating onto modified charcoal–cefoperazone–deoxycholate (mCCD) agar and Preston agar was assessed. The combined results of the detection and enumeration methods revealed a frequency of 70% occurrence of thermotolerant Campylobacter in chicken samples. Campylobacter was enumerated in 60% of the samples, whereas 46% of the samples were positive in the qualitative analysis. Quantitative analysis showed average counts of 3.10 ± 0.15 log(10) CFU/sample. Higher numbers of Campylobacter-positive samples were found using 24-h enrichment before plating onto Preston agar (46%) than onto mCCD agar (2%). The majority of isolated strains were identified as Campylobacter jejuni, and Campylobacter coli was also found but to a lesser extent. Subtyping revealed a clear distinction between strains isolated from different chicken sources. The enriched samples plated onto mCCD agar showed extensive spreading of nonproducing extended-spectrum β-lactamases Proteus mirabilis that hampered the identification of Campylobacter colonies. P. mirabilis strains showed resistance to cefoperazone, trimethoprim, and polymyxin B present in broth and plate media used and were inhibited by rifampicin present in Preston agar. The results underline the effect of the spread of contaminant strains on Campylobacter cultures, which might be prevented using a recently revised International Organization for Standardization method for qualitative analysis of chicken. Elsevier 2020-03-19 /pmc/articles/PMC7597543/ /pubmed/32359606 http://dx.doi.org/10.1016/j.psj.2019.12.061 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Microbiology and Food Safety dos Santos Pozza, Jenifer Voss-Rech, Daiane dos Santos Lopes, Letícia Silveira Luiz Vaz, Clarissa Research Note: A baseline survey of thermotolerant Campylobacter in retail chicken in southern Brazil |
title | Research Note: A baseline survey of thermotolerant Campylobacter in retail chicken in southern Brazil |
title_full | Research Note: A baseline survey of thermotolerant Campylobacter in retail chicken in southern Brazil |
title_fullStr | Research Note: A baseline survey of thermotolerant Campylobacter in retail chicken in southern Brazil |
title_full_unstemmed | Research Note: A baseline survey of thermotolerant Campylobacter in retail chicken in southern Brazil |
title_short | Research Note: A baseline survey of thermotolerant Campylobacter in retail chicken in southern Brazil |
title_sort | research note: a baseline survey of thermotolerant campylobacter in retail chicken in southern brazil |
topic | Microbiology and Food Safety |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7597543/ https://www.ncbi.nlm.nih.gov/pubmed/32359606 http://dx.doi.org/10.1016/j.psj.2019.12.061 |
work_keys_str_mv | AT dossantospozzajenifer researchnoteabaselinesurveyofthermotolerantcampylobacterinretailchickeninsouthernbrazil AT vossrechdaiane researchnoteabaselinesurveyofthermotolerantcampylobacterinretailchickeninsouthernbrazil AT dossantoslopesleticia researchnoteabaselinesurveyofthermotolerantcampylobacterinretailchickeninsouthernbrazil AT silveiraluizvazclarissa researchnoteabaselinesurveyofthermotolerantcampylobacterinretailchickeninsouthernbrazil |