Cargando…
The Absence of NLRP3-inflammasome Modulates Hepatic Fibrosis Progression, Lipid Metabolism, and Inflammation in KO NLRP3 Mice during Aging
Aging is associated with metabolic changes and low-grade inflammation in several organs, which may be due to NLRP3 inflammasome activation. Methods: Here, we asked whether age-related liver changes such as lipid metabolism and fibrosis are reduced in aged mice lacking the NLRP3 inflammasome. We repo...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7598267/ https://www.ncbi.nlm.nih.gov/pubmed/32977490 http://dx.doi.org/10.3390/cells9102148 |
_version_ | 1783602556892610560 |
---|---|
author | Gallego, Paloma Castejón-Vega, Beatriz del Campo, José A. Cordero, Mario D. |
author_facet | Gallego, Paloma Castejón-Vega, Beatriz del Campo, José A. Cordero, Mario D. |
author_sort | Gallego, Paloma |
collection | PubMed |
description | Aging is associated with metabolic changes and low-grade inflammation in several organs, which may be due to NLRP3 inflammasome activation. Methods: Here, we asked whether age-related liver changes such as lipid metabolism and fibrosis are reduced in aged mice lacking the NLRP3 inflammasome. We report reduced protein levels of lipid markers (MTP, FASN, DGAT1), SOD activity, oxidative stress marker PTPRG, and the fibrotic markers TPM2β, COL1-α1 associated with increased GATA4, in NLRP3 deficient mice. Fibrotic, lipid, and oxidative reduction in liver tissues of mice was more pronounced in those old KO NLRP3 mice than in the younger ones, despite their greater liver damage. These results suggest that absence of the NLRP3 inflammasome attenuates age-related liver fibrotic pathology in mice, suggesting that pharmacological targeting may be beneficial. |
format | Online Article Text |
id | pubmed-7598267 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75982672020-10-31 The Absence of NLRP3-inflammasome Modulates Hepatic Fibrosis Progression, Lipid Metabolism, and Inflammation in KO NLRP3 Mice during Aging Gallego, Paloma Castejón-Vega, Beatriz del Campo, José A. Cordero, Mario D. Cells Article Aging is associated with metabolic changes and low-grade inflammation in several organs, which may be due to NLRP3 inflammasome activation. Methods: Here, we asked whether age-related liver changes such as lipid metabolism and fibrosis are reduced in aged mice lacking the NLRP3 inflammasome. We report reduced protein levels of lipid markers (MTP, FASN, DGAT1), SOD activity, oxidative stress marker PTPRG, and the fibrotic markers TPM2β, COL1-α1 associated with increased GATA4, in NLRP3 deficient mice. Fibrotic, lipid, and oxidative reduction in liver tissues of mice was more pronounced in those old KO NLRP3 mice than in the younger ones, despite their greater liver damage. These results suggest that absence of the NLRP3 inflammasome attenuates age-related liver fibrotic pathology in mice, suggesting that pharmacological targeting may be beneficial. MDPI 2020-09-23 /pmc/articles/PMC7598267/ /pubmed/32977490 http://dx.doi.org/10.3390/cells9102148 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gallego, Paloma Castejón-Vega, Beatriz del Campo, José A. Cordero, Mario D. The Absence of NLRP3-inflammasome Modulates Hepatic Fibrosis Progression, Lipid Metabolism, and Inflammation in KO NLRP3 Mice during Aging |
title | The Absence of NLRP3-inflammasome Modulates Hepatic Fibrosis Progression, Lipid Metabolism, and Inflammation in KO NLRP3 Mice during Aging |
title_full | The Absence of NLRP3-inflammasome Modulates Hepatic Fibrosis Progression, Lipid Metabolism, and Inflammation in KO NLRP3 Mice during Aging |
title_fullStr | The Absence of NLRP3-inflammasome Modulates Hepatic Fibrosis Progression, Lipid Metabolism, and Inflammation in KO NLRP3 Mice during Aging |
title_full_unstemmed | The Absence of NLRP3-inflammasome Modulates Hepatic Fibrosis Progression, Lipid Metabolism, and Inflammation in KO NLRP3 Mice during Aging |
title_short | The Absence of NLRP3-inflammasome Modulates Hepatic Fibrosis Progression, Lipid Metabolism, and Inflammation in KO NLRP3 Mice during Aging |
title_sort | absence of nlrp3-inflammasome modulates hepatic fibrosis progression, lipid metabolism, and inflammation in ko nlrp3 mice during aging |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7598267/ https://www.ncbi.nlm.nih.gov/pubmed/32977490 http://dx.doi.org/10.3390/cells9102148 |
work_keys_str_mv | AT gallegopaloma theabsenceofnlrp3inflammasomemodulateshepaticfibrosisprogressionlipidmetabolismandinflammationinkonlrp3miceduringaging AT castejonvegabeatriz theabsenceofnlrp3inflammasomemodulateshepaticfibrosisprogressionlipidmetabolismandinflammationinkonlrp3miceduringaging AT delcampojosea theabsenceofnlrp3inflammasomemodulateshepaticfibrosisprogressionlipidmetabolismandinflammationinkonlrp3miceduringaging AT corderomariod theabsenceofnlrp3inflammasomemodulateshepaticfibrosisprogressionlipidmetabolismandinflammationinkonlrp3miceduringaging AT gallegopaloma absenceofnlrp3inflammasomemodulateshepaticfibrosisprogressionlipidmetabolismandinflammationinkonlrp3miceduringaging AT castejonvegabeatriz absenceofnlrp3inflammasomemodulateshepaticfibrosisprogressionlipidmetabolismandinflammationinkonlrp3miceduringaging AT delcampojosea absenceofnlrp3inflammasomemodulateshepaticfibrosisprogressionlipidmetabolismandinflammationinkonlrp3miceduringaging AT corderomariod absenceofnlrp3inflammasomemodulateshepaticfibrosisprogressionlipidmetabolismandinflammationinkonlrp3miceduringaging |