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Dynamic networks observed in the nucleosome core particles couple the histone globular domains with DNA

The dynamics of eukaryotic nucleosomes are essential in gene activity and well regulated by various factors. Here, we elucidated the internal dynamics at multiple timescales for the human histones hH3 and hH4 in the Widom 601 nucleosome core particles (NCP), suggesting that four dynamic networks are...

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Autores principales: Shi, Xiangyan, Prasanna, Chinmayi, Soman, Aghil, Pervushin, Konstantin, Nordenskiöld, Lars
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7599221/
https://www.ncbi.nlm.nih.gov/pubmed/33128005
http://dx.doi.org/10.1038/s42003-020-01369-3
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author Shi, Xiangyan
Prasanna, Chinmayi
Soman, Aghil
Pervushin, Konstantin
Nordenskiöld, Lars
author_facet Shi, Xiangyan
Prasanna, Chinmayi
Soman, Aghil
Pervushin, Konstantin
Nordenskiöld, Lars
author_sort Shi, Xiangyan
collection PubMed
description The dynamics of eukaryotic nucleosomes are essential in gene activity and well regulated by various factors. Here, we elucidated the internal dynamics at multiple timescales for the human histones hH3 and hH4 in the Widom 601 nucleosome core particles (NCP), suggesting that four dynamic networks are formed by the residues exhibiting larger-scale μs-ms motions that extend from the NCP core to the histone tails and DNA. Furthermore, despite possessing highly conserved structural features, histones in the telomeric NCP exhibit enhanced μs-ms dynamics in the globular sites residing at the identified dynamic networks and in a neighboring region. In addition, higher mobility was observed for the N-terminal tails of hH3 and hH4 in the telomeric NCP. The results demonstrate the existence of dynamic networks in nucleosomes, through which the center of the core regions could interactively communicate with histone tails and DNA to potentially propagate epigenetic changes.
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spelling pubmed-75992212020-11-02 Dynamic networks observed in the nucleosome core particles couple the histone globular domains with DNA Shi, Xiangyan Prasanna, Chinmayi Soman, Aghil Pervushin, Konstantin Nordenskiöld, Lars Commun Biol Article The dynamics of eukaryotic nucleosomes are essential in gene activity and well regulated by various factors. Here, we elucidated the internal dynamics at multiple timescales for the human histones hH3 and hH4 in the Widom 601 nucleosome core particles (NCP), suggesting that four dynamic networks are formed by the residues exhibiting larger-scale μs-ms motions that extend from the NCP core to the histone tails and DNA. Furthermore, despite possessing highly conserved structural features, histones in the telomeric NCP exhibit enhanced μs-ms dynamics in the globular sites residing at the identified dynamic networks and in a neighboring region. In addition, higher mobility was observed for the N-terminal tails of hH3 and hH4 in the telomeric NCP. The results demonstrate the existence of dynamic networks in nucleosomes, through which the center of the core regions could interactively communicate with histone tails and DNA to potentially propagate epigenetic changes. Nature Publishing Group UK 2020-10-30 /pmc/articles/PMC7599221/ /pubmed/33128005 http://dx.doi.org/10.1038/s42003-020-01369-3 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Shi, Xiangyan
Prasanna, Chinmayi
Soman, Aghil
Pervushin, Konstantin
Nordenskiöld, Lars
Dynamic networks observed in the nucleosome core particles couple the histone globular domains with DNA
title Dynamic networks observed in the nucleosome core particles couple the histone globular domains with DNA
title_full Dynamic networks observed in the nucleosome core particles couple the histone globular domains with DNA
title_fullStr Dynamic networks observed in the nucleosome core particles couple the histone globular domains with DNA
title_full_unstemmed Dynamic networks observed in the nucleosome core particles couple the histone globular domains with DNA
title_short Dynamic networks observed in the nucleosome core particles couple the histone globular domains with DNA
title_sort dynamic networks observed in the nucleosome core particles couple the histone globular domains with dna
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7599221/
https://www.ncbi.nlm.nih.gov/pubmed/33128005
http://dx.doi.org/10.1038/s42003-020-01369-3
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