Integrative expression vectors with Pgrac promoters for inducer-free overproduction of recombinant proteins in Bacillus subtilis

Inducer-free integrative vectors are often used to create B. subtilis strains for industrial purposes, but employing strong promoters to produce high levels of recombinant proteins in B. subtilis results in high leaky expression that can hamper cloning in Escherichia coli. To overcome the problem, w...

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Detalles Bibliográficos
Autores principales: Tran, Dinh Thi Minh, Phan, Trang Thi Phuong, Doan, Thanh Thi Ngoc, Tran, Thuoc Linh, Schumann, Wolfgang, Nguyen, Hoang Duc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7599426/
https://www.ncbi.nlm.nih.gov/pubmed/33163371
http://dx.doi.org/10.1016/j.btre.2020.e00540

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7599426/
https://www.ncbi.nlm.nih.gov/pubmed/33163371
http://dx.doi.org/10.1016/j.btre.2020.e00540

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