Promising Antibiofilm Agents: Recent Breakthrough against Biofilm Producing Methicillin-Resistant Staphylococcus aureus

Multidrug resistant (MDR) methicillin-resistant Staphylococcus aureus (MRSA) is a superbug pathogen that causes serious diseases. One of the main reasons for the lack of the effectiveness of antibiotic therapy against infections caused by this resistant pathogen is the recalcitrant nature of MRSA bi...

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Autores principales: Abd El-Hamid, Marwa I., Y. El-Naenaeey, El-sayed, M kandeel, Toka, Hegazy, Wael A. H., Mosbah, Rasha A., Nassar, Majed S., Bakhrebah, Muhammed A., Abdulaal, Wesam H., Alhakamy, Nabil A., Bendary, Mahmoud M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7600973/
https://www.ncbi.nlm.nih.gov/pubmed/33022915
http://dx.doi.org/10.3390/antibiotics9100667
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author Abd El-Hamid, Marwa I.
Y. El-Naenaeey, El-sayed
M kandeel, Toka
Hegazy, Wael A. H.
Mosbah, Rasha A.
Nassar, Majed S.
Bakhrebah, Muhammed A.
Abdulaal, Wesam H.
Alhakamy, Nabil A.
Bendary, Mahmoud M.
author_facet Abd El-Hamid, Marwa I.
Y. El-Naenaeey, El-sayed
M kandeel, Toka
Hegazy, Wael A. H.
Mosbah, Rasha A.
Nassar, Majed S.
Bakhrebah, Muhammed A.
Abdulaal, Wesam H.
Alhakamy, Nabil A.
Bendary, Mahmoud M.
author_sort Abd El-Hamid, Marwa I.
collection PubMed
description Multidrug resistant (MDR) methicillin-resistant Staphylococcus aureus (MRSA) is a superbug pathogen that causes serious diseases. One of the main reasons for the lack of the effectiveness of antibiotic therapy against infections caused by this resistant pathogen is the recalcitrant nature of MRSA biofilms, which results in an increasingly serious situation worldwide. Consequently, the development of innovative biofilm inhibitors is urgently needed to control the biofilm formation by this pathogen. In this work, we thus sought to evaluate the biofilm inhibiting ability of some promising antibiofilm agents such as zinc oxide nanoparticles (Zno NPs), proteinase K, and hamamelitannin (HAM) in managing the MRSA biofilms. Different phenotypic and genotypic methods were used to identify the biofilm producing MDR MRSA isolates and the antibiofilm/antimicrobial activities of the used promising agents. Our study demonstrated strong antibiofilm activities of ZnO NPs, proteinase K, and HAM against MRSA biofilms along with their transcriptional modulation of biofilm (intercellular adhesion A, icaA) and quorum sensing (QS) (agr) genes. Interestingly, only ZnO NPs showed a powerful antimicrobial activity against this pathogen. Collectively, we observed overall positive correlations between the biofilm production and the antimicrobial resistance/agr genotypes II and IV. Meanwhile, there was no significant correlation between the toxin genes and the biofilm production. The ZnO NPs were recommended to be used alone as potent antimicrobial and antibiofilm agents against MDR MRSA and their biofilm-associated diseases. On the other hand, proteinase-K and HAM can be co-administrated with other antimicrobial agents to manage such types of infections.
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spelling pubmed-76009732020-11-01 Promising Antibiofilm Agents: Recent Breakthrough against Biofilm Producing Methicillin-Resistant Staphylococcus aureus Abd El-Hamid, Marwa I. Y. El-Naenaeey, El-sayed M kandeel, Toka Hegazy, Wael A. H. Mosbah, Rasha A. Nassar, Majed S. Bakhrebah, Muhammed A. Abdulaal, Wesam H. Alhakamy, Nabil A. Bendary, Mahmoud M. Antibiotics (Basel) Article Multidrug resistant (MDR) methicillin-resistant Staphylococcus aureus (MRSA) is a superbug pathogen that causes serious diseases. One of the main reasons for the lack of the effectiveness of antibiotic therapy against infections caused by this resistant pathogen is the recalcitrant nature of MRSA biofilms, which results in an increasingly serious situation worldwide. Consequently, the development of innovative biofilm inhibitors is urgently needed to control the biofilm formation by this pathogen. In this work, we thus sought to evaluate the biofilm inhibiting ability of some promising antibiofilm agents such as zinc oxide nanoparticles (Zno NPs), proteinase K, and hamamelitannin (HAM) in managing the MRSA biofilms. Different phenotypic and genotypic methods were used to identify the biofilm producing MDR MRSA isolates and the antibiofilm/antimicrobial activities of the used promising agents. Our study demonstrated strong antibiofilm activities of ZnO NPs, proteinase K, and HAM against MRSA biofilms along with their transcriptional modulation of biofilm (intercellular adhesion A, icaA) and quorum sensing (QS) (agr) genes. Interestingly, only ZnO NPs showed a powerful antimicrobial activity against this pathogen. Collectively, we observed overall positive correlations between the biofilm production and the antimicrobial resistance/agr genotypes II and IV. Meanwhile, there was no significant correlation between the toxin genes and the biofilm production. The ZnO NPs were recommended to be used alone as potent antimicrobial and antibiofilm agents against MDR MRSA and their biofilm-associated diseases. On the other hand, proteinase-K and HAM can be co-administrated with other antimicrobial agents to manage such types of infections. MDPI 2020-10-03 /pmc/articles/PMC7600973/ /pubmed/33022915 http://dx.doi.org/10.3390/antibiotics9100667 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Abd El-Hamid, Marwa I.
Y. El-Naenaeey, El-sayed
M kandeel, Toka
Hegazy, Wael A. H.
Mosbah, Rasha A.
Nassar, Majed S.
Bakhrebah, Muhammed A.
Abdulaal, Wesam H.
Alhakamy, Nabil A.
Bendary, Mahmoud M.
Promising Antibiofilm Agents: Recent Breakthrough against Biofilm Producing Methicillin-Resistant Staphylococcus aureus
title Promising Antibiofilm Agents: Recent Breakthrough against Biofilm Producing Methicillin-Resistant Staphylococcus aureus
title_full Promising Antibiofilm Agents: Recent Breakthrough against Biofilm Producing Methicillin-Resistant Staphylococcus aureus
title_fullStr Promising Antibiofilm Agents: Recent Breakthrough against Biofilm Producing Methicillin-Resistant Staphylococcus aureus
title_full_unstemmed Promising Antibiofilm Agents: Recent Breakthrough against Biofilm Producing Methicillin-Resistant Staphylococcus aureus
title_short Promising Antibiofilm Agents: Recent Breakthrough against Biofilm Producing Methicillin-Resistant Staphylococcus aureus
title_sort promising antibiofilm agents: recent breakthrough against biofilm producing methicillin-resistant staphylococcus aureus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7600973/
https://www.ncbi.nlm.nih.gov/pubmed/33022915
http://dx.doi.org/10.3390/antibiotics9100667
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