Cargando…
Inactivation of Multi-Drug Resistant Non-Typhoidal Salmonella and Wild-Type Escherichia coli STEC Using Organic Acids: A Potential Alternative to the Food Industry
Salmonella and Escherichia coli are the main bacterial species involved in food outbreaks worldwide. Recent reports showed that chemical sanitizers commonly used to control these pathogens could induce antibiotic resistance. Therefore, this study aimed to describe the efficiency of chemical sanitize...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7602699/ https://www.ncbi.nlm.nih.gov/pubmed/33081230 http://dx.doi.org/10.3390/pathogens9100849 |
_version_ | 1783603743854428160 |
---|---|
author | Castro, Vinicius Silva Mutz, Yhan da Silva Rosario, Denes Kaic Alves Cunha-Neto, Adelino Figueiredo, Eduardo Eustáquio de Souza Conte-Junior, Carlos Adam |
author_facet | Castro, Vinicius Silva Mutz, Yhan da Silva Rosario, Denes Kaic Alves Cunha-Neto, Adelino Figueiredo, Eduardo Eustáquio de Souza Conte-Junior, Carlos Adam |
author_sort | Castro, Vinicius Silva |
collection | PubMed |
description | Salmonella and Escherichia coli are the main bacterial species involved in food outbreaks worldwide. Recent reports showed that chemical sanitizers commonly used to control these pathogens could induce antibiotic resistance. Therefore, this study aimed to describe the efficiency of chemical sanitizers and organic acids when inactivating wild and clinical strains of Salmonella and E. coli, targeting a 4-log reduction. To achieve this goal, three methods were applied. (i) Disk-diffusion challenge for organic acids. (ii) Determination of MIC for two acids (acetic and lactic), as well as two sanitizers (quaternary compound and sodium hypochlorite). (iii) The development of inactivation models from the previously defined concentrations. In disk-diffusion, the results indicated that wild strains have higher resistance potential when compared to clinical strains. Regarding the models, quaternary ammonium and lactic acid showed a linear pattern of inactivation, while sodium hypochlorite had a linear pattern with tail dispersion, and acetic acid has Weibull dispersion to E. coli. The concentration to 4-log reduction differed from Salmonella and E. coli in acetic acid and sodium hypochlorite. The use of organic acids is an alternative method for antimicrobial control. Our study indicates the levels of organic acids and sanitizers to be used in the inactivation of emerging foodborne pathogens. |
format | Online Article Text |
id | pubmed-7602699 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76026992020-11-01 Inactivation of Multi-Drug Resistant Non-Typhoidal Salmonella and Wild-Type Escherichia coli STEC Using Organic Acids: A Potential Alternative to the Food Industry Castro, Vinicius Silva Mutz, Yhan da Silva Rosario, Denes Kaic Alves Cunha-Neto, Adelino Figueiredo, Eduardo Eustáquio de Souza Conte-Junior, Carlos Adam Pathogens Article Salmonella and Escherichia coli are the main bacterial species involved in food outbreaks worldwide. Recent reports showed that chemical sanitizers commonly used to control these pathogens could induce antibiotic resistance. Therefore, this study aimed to describe the efficiency of chemical sanitizers and organic acids when inactivating wild and clinical strains of Salmonella and E. coli, targeting a 4-log reduction. To achieve this goal, three methods were applied. (i) Disk-diffusion challenge for organic acids. (ii) Determination of MIC for two acids (acetic and lactic), as well as two sanitizers (quaternary compound and sodium hypochlorite). (iii) The development of inactivation models from the previously defined concentrations. In disk-diffusion, the results indicated that wild strains have higher resistance potential when compared to clinical strains. Regarding the models, quaternary ammonium and lactic acid showed a linear pattern of inactivation, while sodium hypochlorite had a linear pattern with tail dispersion, and acetic acid has Weibull dispersion to E. coli. The concentration to 4-log reduction differed from Salmonella and E. coli in acetic acid and sodium hypochlorite. The use of organic acids is an alternative method for antimicrobial control. Our study indicates the levels of organic acids and sanitizers to be used in the inactivation of emerging foodborne pathogens. MDPI 2020-10-16 /pmc/articles/PMC7602699/ /pubmed/33081230 http://dx.doi.org/10.3390/pathogens9100849 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Castro, Vinicius Silva Mutz, Yhan da Silva Rosario, Denes Kaic Alves Cunha-Neto, Adelino Figueiredo, Eduardo Eustáquio de Souza Conte-Junior, Carlos Adam Inactivation of Multi-Drug Resistant Non-Typhoidal Salmonella and Wild-Type Escherichia coli STEC Using Organic Acids: A Potential Alternative to the Food Industry |
title | Inactivation of Multi-Drug Resistant Non-Typhoidal Salmonella and Wild-Type Escherichia coli STEC Using Organic Acids: A Potential Alternative to the Food Industry |
title_full | Inactivation of Multi-Drug Resistant Non-Typhoidal Salmonella and Wild-Type Escherichia coli STEC Using Organic Acids: A Potential Alternative to the Food Industry |
title_fullStr | Inactivation of Multi-Drug Resistant Non-Typhoidal Salmonella and Wild-Type Escherichia coli STEC Using Organic Acids: A Potential Alternative to the Food Industry |
title_full_unstemmed | Inactivation of Multi-Drug Resistant Non-Typhoidal Salmonella and Wild-Type Escherichia coli STEC Using Organic Acids: A Potential Alternative to the Food Industry |
title_short | Inactivation of Multi-Drug Resistant Non-Typhoidal Salmonella and Wild-Type Escherichia coli STEC Using Organic Acids: A Potential Alternative to the Food Industry |
title_sort | inactivation of multi-drug resistant non-typhoidal salmonella and wild-type escherichia coli stec using organic acids: a potential alternative to the food industry |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7602699/ https://www.ncbi.nlm.nih.gov/pubmed/33081230 http://dx.doi.org/10.3390/pathogens9100849 |
work_keys_str_mv | AT castroviniciussilva inactivationofmultidrugresistantnontyphoidalsalmonellaandwildtypeescherichiacolistecusingorganicacidsapotentialalternativetothefoodindustry AT mutzyhandasilva inactivationofmultidrugresistantnontyphoidalsalmonellaandwildtypeescherichiacolistecusingorganicacidsapotentialalternativetothefoodindustry AT rosariodeneskaicalves inactivationofmultidrugresistantnontyphoidalsalmonellaandwildtypeescherichiacolistecusingorganicacidsapotentialalternativetothefoodindustry AT cunhanetoadelino inactivationofmultidrugresistantnontyphoidalsalmonellaandwildtypeescherichiacolistecusingorganicacidsapotentialalternativetothefoodindustry AT figueiredoeduardoeustaquiodesouza inactivationofmultidrugresistantnontyphoidalsalmonellaandwildtypeescherichiacolistecusingorganicacidsapotentialalternativetothefoodindustry AT contejuniorcarlosadam inactivationofmultidrugresistantnontyphoidalsalmonellaandwildtypeescherichiacolistecusingorganicacidsapotentialalternativetothefoodindustry |