Changes in the Honeybee Antioxidant System after 12 h of Exposure to Electromagnetic Field Frequency of 50 Hz and Variable Intensity

SIMPLE SUMMARY: The honeybee is one of the most important links in the food production chain. In the environment of bee life, there are many threats that they have to face. Among them, we can distinguish pesticides, climate change, and predators. The intensive development of technology resulted in filling the natural environment with E-field of various frequencies and intensities. The study investigated the effect of the E-field with a frequency of 50 Hz at various intensities on the antioxidant system of the honeybee. The exposure of the bees lasted 12 h. The activity of the antioxidant system was investigated in hemolymph collected from young bees. Research has been undertaken in this direction because research by other authors has taken years to stimulate this system through the E-field. The superoxide dismutase (SOD), Catalase (CAT), and total antioxidant potential (FRAP) activity as major antioxidant enzymes were characterized. Research showed changes in the activity of SOD, CAT, and FRAP in all tested groups compared to the control group. The study of E-field appearing in the life of a honeybee enables an understanding of the impact of this factor on its functioning because food production depends on the integrity of this pollinator. ABSTRACT: In recent years, on a global scale, more and more reports of a phenomenon called CCD (Colony Collapse Disorder) have been reported. In addition to pesticides, diseases, and other environmental stressors, electromagnetic fields are also mentioned as one of the possible causes of CCD. One of the body’s first lines of defense against harmful factors is the antioxidant system. We hypothesized that electromagnetic field upregulate the activity of SOD (superoxide dismutase), CAT (catalases), and changed FRAP (total antioxidant potential) in honeybee hemolymph. In our research, 12 h bee’s exposure to E-field was analyzed to determine changes in the antioxidant system. The frequency of 50 Hz and various intensities were used: 5.0 kV/m, 11.5 kV/m, 23.0 kV/m, and 34.5 kV/m. Superoxide dismutase was characterized by four times higher activity in the study groups as compared to the control group. Catalase activity in all groups was characterized by statistically significantly different activity between the groups. The highest activity was recorded in the 34.5 kV/m group. The lowest activity was recorded in the 11.5 kV/m group. A relationship was found between different E-field intensities and changes in the antioxidant system.