Robust Cell-Free Expression of Sub-Pathological and Pathological Huntingtin Exon-1 for NMR Studies. General Approaches for the Isotopic Labeling of Low-Complexity Proteins

The high-resolution structural study of huntingtin exon-1 (HttEx1) has long been hampered by its intrinsic properties. In addition to being prone to aggregate, HttEx1 contains low-complexity regions (LCRs) and is intrinsically disordered, ruling out several standard structural biology approaches. He...

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Detalles Bibliográficos
Autores principales: Morató, Anna, Elena-Real, Carlos A., Popovic, Matija, Fournet, Aurélie, Zhang, Karen, Allemand, Frédéric, Sibille, Nathalie, Urbanek, Annika, Bernadó, Pau
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7603387/
https://www.ncbi.nlm.nih.gov/pubmed/33086646
http://dx.doi.org/10.3390/biom10101458

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7603387/
https://www.ncbi.nlm.nih.gov/pubmed/33086646
http://dx.doi.org/10.3390/biom10101458

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