Pooled analysis of radiation hybrids identifies loci for growth and drug action in mammalian cells

Genetic screens in mammalian cells commonly focus on loss-of-function approaches. To evaluate the phenotypic consequences of extra gene copies, we used bulk segregant analysis (BSA) of radiation hybrid (RH) cells. We constructed six pools of RH cells, each consisting of ∼2500 independent clones, and...

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Detalles Bibliográficos
Autores principales: Khan, Arshad H., Lin, Andy, Wang, Richard T., Bloom, Joshua S., Lange, Kenneth, Smith, Desmond J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2020
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7605260/
https://www.ncbi.nlm.nih.gov/pubmed/32878976
http://dx.doi.org/10.1101/gr.262204.120

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7605260/
https://www.ncbi.nlm.nih.gov/pubmed/32878976
http://dx.doi.org/10.1101/gr.262204.120

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