Cloning short DNA into plasmids by one‐step PCR

BACKGROUND: Plasmid construction of small fragments of interest (such as insertion of small fragment marker genes, expression of shRNA, siRNA, etc) is the basis of many biomolecular experiments. Here, we describe a method to clone short DNA into vectors by polymerase chain reaction (PCR), named one‐...

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Detalles Bibliográficos
Autores principales: Tao, Cheng‐Cheng, Yang, Ying, Li, Fang, Qiao, Ling, Wu, Yue, Sun, Xiao‐Dong, Zhang, Yuan‐Yuan, Li, Chang‐Long
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons Australia, Ltd 2020
Materias:
Technological Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7605993/
https://www.ncbi.nlm.nih.gov/pubmed/33015950
http://dx.doi.org/10.1111/1759-7714.13660

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7605993/
https://www.ncbi.nlm.nih.gov/pubmed/33015950
http://dx.doi.org/10.1111/1759-7714.13660

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