Thermodynamic analysis of cooperative ligand binding by the ATP-binding DNA aptamer indicates a population-shift binding mechanism

The ATP-binding DNA aptamer is often used as a model system for developing new aptamer-based biosensor methods. This aptamer follows a structure-switching binding mechanism and is unusual in that it binds two copies of its ligand. We have used isothermal titration calorimetry methods to study the bi...

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Detalles Bibliográficos
Autores principales: Slavkovic, Sladjana, Zhu, Yanrui, Churcher, Zachary R., Shoara, Aron A., Johnson, Anne E., Johnson, Philip E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7609719/
https://www.ncbi.nlm.nih.gov/pubmed/33144644
http://dx.doi.org/10.1038/s41598-020-76002-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7609719/
https://www.ncbi.nlm.nih.gov/pubmed/33144644
http://dx.doi.org/10.1038/s41598-020-76002-8

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