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Targeted mutagenesis of multiple chromosomal regions in microbes
Directed evolution allows the effective engineering of proteins, biosynthetic pathways, and cellular functions. Traditional plasmid-based methods generally subject one or occasionally multiple genes-of-interest to mutagenesis, require time-consuming manual interventions, and the genes that are subje...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7613694/ https://www.ncbi.nlm.nih.gov/pubmed/32599531 http://dx.doi.org/10.1016/j.mib.2020.05.010 |