An improved imaging system that corrects MS2-induced RNA destabilization

The MS2-MCP imaging system is widely used to study mRNA spatial distribution in living cells. Here, we report that the MS2-MCP system destabilizes some tagged mRNAs by targeting them to the nonsense-mediated mRNA decay pathway. We introduce an improved version, which counteracts this effect by incre...

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Detalles Bibliográficos
Autores principales: Li, Weihan, Maekiniemi, Anna, Sato, Hanae, Osman, Christof, Singer, Robert H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7613886/
https://www.ncbi.nlm.nih.gov/pubmed/36357695
http://dx.doi.org/10.1038/s41592-022-01658-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7613886/
https://www.ncbi.nlm.nih.gov/pubmed/36357695
http://dx.doi.org/10.1038/s41592-022-01658-1

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