An improved imaging system that corrects MS2-induced RNA destabilization

The MS2-MCP imaging system is widely used to study mRNA spatial distribution in living cells. Here, we report that the MS2-MCP system destabilizes some tagged mRNAs by targeting them to the nonsense-mediated mRNA decay pathway. We introduce an improved version, which counteracts this effect by incre...

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Detalles Bibliográficos
Autores principales: Li, Weihan, Maekiniemi, Anna, Sato, Hanae, Osman, Christof, Singer, Robert H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7613886/
https://www.ncbi.nlm.nih.gov/pubmed/36357695
http://dx.doi.org/10.1038/s41592-022-01658-1
Descripción
Sumario:The MS2-MCP imaging system is widely used to study mRNA spatial distribution in living cells. Here, we report that the MS2-MCP system destabilizes some tagged mRNAs by targeting them to the nonsense-mediated mRNA decay pathway. We introduce an improved version, which counteracts this effect by increasing the efficiency of translation termination of the tagged mRNAs. Improved versions were developed for both yeast and mammalian systems.

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