An improved imaging system that corrects MS2-induced RNA destabilization

The MS2-MCP imaging system is widely used to study mRNA spatial distribution in living cells. Here, we report that the MS2-MCP system destabilizes some tagged mRNAs by targeting them to the nonsense-mediated mRNA decay pathway. We introduce an improved version, which counteracts this effect by incre...

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Detalles Bibliográficos
Autores principales: Li, Weihan, Maekiniemi, Anna, Sato, Hanae, Osman, Christof, Singer, Robert H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7613886/
https://www.ncbi.nlm.nih.gov/pubmed/36357695
http://dx.doi.org/10.1038/s41592-022-01658-1
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author Li, Weihan
Maekiniemi, Anna
Sato, Hanae
Osman, Christof
Singer, Robert H.
author_facet Li, Weihan
Maekiniemi, Anna
Sato, Hanae
Osman, Christof
Singer, Robert H.
author_sort Li, Weihan
collection PubMed
description The MS2-MCP imaging system is widely used to study mRNA spatial distribution in living cells. Here, we report that the MS2-MCP system destabilizes some tagged mRNAs by targeting them to the nonsense-mediated mRNA decay pathway. We introduce an improved version, which counteracts this effect by increasing the efficiency of translation termination of the tagged mRNAs. Improved versions were developed for both yeast and mammalian systems.
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spelling pubmed-76138862023-05-10 An improved imaging system that corrects MS2-induced RNA destabilization Li, Weihan Maekiniemi, Anna Sato, Hanae Osman, Christof Singer, Robert H. Nat Methods Article The MS2-MCP imaging system is widely used to study mRNA spatial distribution in living cells. Here, we report that the MS2-MCP system destabilizes some tagged mRNAs by targeting them to the nonsense-mediated mRNA decay pathway. We introduce an improved version, which counteracts this effect by increasing the efficiency of translation termination of the tagged mRNAs. Improved versions were developed for both yeast and mammalian systems. 2022-12 2022-11-10 /pmc/articles/PMC7613886/ /pubmed/36357695 http://dx.doi.org/10.1038/s41592-022-01658-1 Text en https://www.springernature.com/gp/open-research/policies/accepted-manuscript-termsUsers may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: https://www.springernature.com/gp/open-research/policies/accepted-manuscript-terms
spellingShingle Article
Li, Weihan
Maekiniemi, Anna
Sato, Hanae
Osman, Christof
Singer, Robert H.
An improved imaging system that corrects MS2-induced RNA destabilization
title An improved imaging system that corrects MS2-induced RNA destabilization
title_full An improved imaging system that corrects MS2-induced RNA destabilization
title_fullStr An improved imaging system that corrects MS2-induced RNA destabilization
title_full_unstemmed An improved imaging system that corrects MS2-induced RNA destabilization
title_short An improved imaging system that corrects MS2-induced RNA destabilization
title_sort improved imaging system that corrects ms2-induced rna destabilization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7613886/
https://www.ncbi.nlm.nih.gov/pubmed/36357695
http://dx.doi.org/10.1038/s41592-022-01658-1
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