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An improved imaging system that corrects MS2-induced RNA destabilization
The MS2-MCP imaging system is widely used to study mRNA spatial distribution in living cells. Here, we report that the MS2-MCP system destabilizes some tagged mRNAs by targeting them to the nonsense-mediated mRNA decay pathway. We introduce an improved version, which counteracts this effect by incre...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7613886/ https://www.ncbi.nlm.nih.gov/pubmed/36357695 http://dx.doi.org/10.1038/s41592-022-01658-1 |