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A sensitive and less cytotoxic assay for identification of proliferating T cells based on bioorthogonally-functionalized uridine analogue

Quantitative detection of T cell proliferation is an important readout in immunology research, as it is one of the hallmarks of T cell activation. Fluorescence-based methods for T cell proliferation mostly rely on the usage of probes that non-specifically conjugate to free primary amine groups in ce...

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Detalles Bibliográficos
Autores principales: Stempels, F.C., de Wit, A.S., Swierstra, M.S., Maassen, S., Bianchi, F., van den Bogaart, G., Baranov, M.V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7614694/
https://www.ncbi.nlm.nih.gov/pubmed/35074315
http://dx.doi.org/10.1016/j.jim.2022.113228