High-Throughput Quantitative RT-PCR in Single and Bulk C. elegans Samples Using Nanofluidic Technology

This paper presents a high-throughput reverse transcription quantitative PCR (RT-qPCR) assay for Caenorhabditis elegans that is fast, robust, and highly sensitive. This protocol obtains precise measurements of gene expression from single worms or from bulk samples. The protocol presented here provid...

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Detalles Bibliográficos
Autores principales: Chauve, Laetitia, Le Pen, Jérémie, Hodge, Francesca, Todtenhaupt, Pia, Biggins, Laura, Miska, Eric A., Andrews, Simon, Casanueva, Olivia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7614925/
https://www.ncbi.nlm.nih.gov/pubmed/32538915
http://dx.doi.org/10.3791/61132

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7614925/
https://www.ncbi.nlm.nih.gov/pubmed/32538915
http://dx.doi.org/10.3791/61132

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