Rapid poxvirus engineering using CRISPR/Cas9 as a selection tool

In standard uses of CRISPR/Cas9 technology, the cutting of genomes and their efficient repair are considered to go hand-in-hand to achieve desired genetic changes. This includes the current approach for engineering genomes of large dsDNA viruses. However, for poxviruses we show that Cas9-guide RNA c...

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Detalles Bibliográficos
Autores principales: Gowripalan, Anjali, Smith, Stewart, Stefanovic, Tijana, Tscharke, David C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7641209/
https://www.ncbi.nlm.nih.gov/pubmed/33144673
http://dx.doi.org/10.1038/s42003-020-01374-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7641209/
https://www.ncbi.nlm.nih.gov/pubmed/33144673
http://dx.doi.org/10.1038/s42003-020-01374-6

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