A universal method for the rapid isolation of all known classes of functional silencing small RNAs

Diverse classes of silencing small (s)RNAs operate via ARGONAUTE-family proteins within RNA-induced-silencing-complexes (RISCs). Here, we have streamlined various embodiments of a Q-sepharose-based RISC-purification method that relies on conserved biochemical properties of all ARGONAUTEs. We show, i...

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Detalles Bibliográficos
Autores principales: Grentzinger, Thomas, Oberlin, Stefan, Schott, Gregory, Handler, Dominik, Svozil, Julia, Barragan-Borrero, Veronica, Humbert, Adeline, Duharcourt, Sandra, Brennecke, Julius, Voinnet, Olivier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7641303/
https://www.ncbi.nlm.nih.gov/pubmed/32496553
http://dx.doi.org/10.1093/nar/gkaa472

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7641303/
https://www.ncbi.nlm.nih.gov/pubmed/32496553
http://dx.doi.org/10.1093/nar/gkaa472

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