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Bacterial genome editing by coupling Cre-lox and CRISPR-Cas9 systems

The past decade has been a golden age for microbiology, marked by the discovery of an unprecedented increase in the number of novel bacterial species. Yet gaining biological knowledge of those organisms has not kept pace with sequencing efforts. To unlock this genetic potential there is an urgent ne...

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Detalles Bibliográficos
Autores principales: Liu, Hualan, Robinson, David S., Wu, Zong-Yen, Kuo, Rita, Yoshikuni, Yasuo, Blaby, Ian K., Cheng, Jan-Fang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7641437/
https://www.ncbi.nlm.nih.gov/pubmed/33147260
http://dx.doi.org/10.1371/journal.pone.0241867