RNase H-dependent PCR enables highly specific amplification of antibody variable domains from single B-cells

Immunization-based antibody discovery platforms require robust and effective protocols for the amplification, cloning, expression, and screening of antibodies from large numbers of B-cells in order to effectively capture the diversity of an experienced Ig-repertoire. Multiplex PCR using a series of...

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Detalles Bibliográficos
Autores principales: Crissman, John, Lin, Yuhao, Separa, Kevin, Duquette, Madeleine, Cohen, Michael, Velasquez, Candyd, Cujec, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7643965/
https://www.ncbi.nlm.nih.gov/pubmed/33152031
http://dx.doi.org/10.1371/journal.pone.0241803

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7643965/
https://www.ncbi.nlm.nih.gov/pubmed/33152031
http://dx.doi.org/10.1371/journal.pone.0241803

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