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RNase H-dependent PCR enables highly specific amplification of antibody variable domains from single B-cells
Immunization-based antibody discovery platforms require robust and effective protocols for the amplification, cloning, expression, and screening of antibodies from large numbers of B-cells in order to effectively capture the diversity of an experienced Ig-repertoire. Multiplex PCR using a series of...
Autores principales: | Crissman, John, Lin, Yuhao, Separa, Kevin, Duquette, Madeleine, Cohen, Michael, Velasquez, Candyd, Cujec, Thomas |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7643965/ https://www.ncbi.nlm.nih.gov/pubmed/33152031 http://dx.doi.org/10.1371/journal.pone.0241803 |
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