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Protection of outbred mice against a vaginal challenge by a Chlamydia trachomatis serovar E recombinant major outer membrane protein vaccine is dependent on phosphate substitution in the adjuvant

Chlamydia trachomatis is the most common bacterial sexually-transmitted pathogen for which there is no vaccine. We previously demonstrated that the degree of phosphate substitution in an aluminum hydroxide adjuvant in a TLR-4-based C. trachomatis serovar E (Ser E) recombinant major outer membrane pr...

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Autores principales: Pal, Sukumar, Ausar, Salvador Fernando, Tifrea, Delia F., Cheng, Chunmei, Gallichan, Scott, Sanchez, Violette, de la Maza, Luis M., Visan, Lucian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7644203/
https://www.ncbi.nlm.nih.gov/pubmed/32118511
http://dx.doi.org/10.1080/21645515.2020.1717183
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author Pal, Sukumar
Ausar, Salvador Fernando
Tifrea, Delia F.
Cheng, Chunmei
Gallichan, Scott
Sanchez, Violette
de la Maza, Luis M.
Visan, Lucian
author_facet Pal, Sukumar
Ausar, Salvador Fernando
Tifrea, Delia F.
Cheng, Chunmei
Gallichan, Scott
Sanchez, Violette
de la Maza, Luis M.
Visan, Lucian
author_sort Pal, Sukumar
collection PubMed
description Chlamydia trachomatis is the most common bacterial sexually-transmitted pathogen for which there is no vaccine. We previously demonstrated that the degree of phosphate substitution in an aluminum hydroxide adjuvant in a TLR-4-based C. trachomatis serovar E (Ser E) recombinant major outer membrane protein (rMOMP) formulation had an impact on the induced antibody titers and IFN-γ levels. Here, we have extended these observations using outbreed CD-1 mice immunized with C. trachomatis Ser E rMOMP formulations to evaluate the impact on bacterial challenge. The results confirmed that the rMOMP vaccine containing the adjuvant with the highest phosphate substitution induced the highest neutralizing antibody titers while the formulation with the lowest phosphate substitution induced the highest IFN-γ production. The most robust protection was observed in mice vaccinated with the formulation containing the adjuvant with the lowest phosphate substitution, as shown by the number of mice with positive vaginal cultures, number of positive cultures and number of C. trachomatis inclusion forming units recovered. This is the first report showing that vaccination of an outbred strain of mice with rMOMP induces protection against a vaginal challenge with C. trachomatis.
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spelling pubmed-76442032020-11-13 Protection of outbred mice against a vaginal challenge by a Chlamydia trachomatis serovar E recombinant major outer membrane protein vaccine is dependent on phosphate substitution in the adjuvant Pal, Sukumar Ausar, Salvador Fernando Tifrea, Delia F. Cheng, Chunmei Gallichan, Scott Sanchez, Violette de la Maza, Luis M. Visan, Lucian Hum Vaccin Immunother Research Paper Chlamydia trachomatis is the most common bacterial sexually-transmitted pathogen for which there is no vaccine. We previously demonstrated that the degree of phosphate substitution in an aluminum hydroxide adjuvant in a TLR-4-based C. trachomatis serovar E (Ser E) recombinant major outer membrane protein (rMOMP) formulation had an impact on the induced antibody titers and IFN-γ levels. Here, we have extended these observations using outbreed CD-1 mice immunized with C. trachomatis Ser E rMOMP formulations to evaluate the impact on bacterial challenge. The results confirmed that the rMOMP vaccine containing the adjuvant with the highest phosphate substitution induced the highest neutralizing antibody titers while the formulation with the lowest phosphate substitution induced the highest IFN-γ production. The most robust protection was observed in mice vaccinated with the formulation containing the adjuvant with the lowest phosphate substitution, as shown by the number of mice with positive vaginal cultures, number of positive cultures and number of C. trachomatis inclusion forming units recovered. This is the first report showing that vaccination of an outbred strain of mice with rMOMP induces protection against a vaginal challenge with C. trachomatis. Taylor & Francis 2020-03-02 /pmc/articles/PMC7644203/ /pubmed/32118511 http://dx.doi.org/10.1080/21645515.2020.1717183 Text en © 2020 The Author(s). Published with license by Taylor & Francis Group, LLC. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Pal, Sukumar
Ausar, Salvador Fernando
Tifrea, Delia F.
Cheng, Chunmei
Gallichan, Scott
Sanchez, Violette
de la Maza, Luis M.
Visan, Lucian
Protection of outbred mice against a vaginal challenge by a Chlamydia trachomatis serovar E recombinant major outer membrane protein vaccine is dependent on phosphate substitution in the adjuvant
title Protection of outbred mice against a vaginal challenge by a Chlamydia trachomatis serovar E recombinant major outer membrane protein vaccine is dependent on phosphate substitution in the adjuvant
title_full Protection of outbred mice against a vaginal challenge by a Chlamydia trachomatis serovar E recombinant major outer membrane protein vaccine is dependent on phosphate substitution in the adjuvant
title_fullStr Protection of outbred mice against a vaginal challenge by a Chlamydia trachomatis serovar E recombinant major outer membrane protein vaccine is dependent on phosphate substitution in the adjuvant
title_full_unstemmed Protection of outbred mice against a vaginal challenge by a Chlamydia trachomatis serovar E recombinant major outer membrane protein vaccine is dependent on phosphate substitution in the adjuvant
title_short Protection of outbred mice against a vaginal challenge by a Chlamydia trachomatis serovar E recombinant major outer membrane protein vaccine is dependent on phosphate substitution in the adjuvant
title_sort protection of outbred mice against a vaginal challenge by a chlamydia trachomatis serovar e recombinant major outer membrane protein vaccine is dependent on phosphate substitution in the adjuvant
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7644203/
https://www.ncbi.nlm.nih.gov/pubmed/32118511
http://dx.doi.org/10.1080/21645515.2020.1717183
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