Estimates of Rel(Seq), Mesh1, and SAH(Mex) Hydrolysis of (p)ppGpp and (p)ppApp by Thin Layer Chromatography and NADP/NADH Coupled Assays

The Mesh1 class of hydrolases found in bacteria, metazoans and humans was discovered as able to cleave an intact pyrophosphate residue esterified on the 3′hydroxyl of (p)ppGpp in a Mn(2+) dependent reaction. Here, thin layer chromatography (TLC) qualitative evidence is presented indicating the subst...

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Detalles Bibliográficos
Autores principales: Potrykus, Katarzyna, Thomas, Nathan E., Bruhn-Olszewska, Bożena, Sobala, Michał, Dylewski, Maciej, James, Tamara, Cashel, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7644958/
https://www.ncbi.nlm.nih.gov/pubmed/33193211
http://dx.doi.org/10.3389/fmicb.2020.581271

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7644958/
https://www.ncbi.nlm.nih.gov/pubmed/33193211
http://dx.doi.org/10.3389/fmicb.2020.581271

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