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Estimates of Rel(Seq), Mesh1, and SAH(Mex) Hydrolysis of (p)ppGpp and (p)ppApp by Thin Layer Chromatography and NADP/NADH Coupled Assays
The Mesh1 class of hydrolases found in bacteria, metazoans and humans was discovered as able to cleave an intact pyrophosphate residue esterified on the 3′hydroxyl of (p)ppGpp in a Mn(2+) dependent reaction. Here, thin layer chromatography (TLC) qualitative evidence is presented indicating the subst...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Frontiers Media S.A.
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7644958/ https://www.ncbi.nlm.nih.gov/pubmed/33193211 http://dx.doi.org/10.3389/fmicb.2020.581271 |