Arrayed CRISPRi and quantitative imaging describe the morphotypic landscape of essential mycobacterial genes

Mycobacterium tuberculosis possesses a large number of genes of unknown or predicted function, undermining fundamental understanding of pathogenicity and drug susceptibility. To address this challenge, we developed a high-throughput functional genomics approach combining inducible CRISPR-interferenc...

Descripción completa

Detalles Bibliográficos
Autores principales: de Wet, Timothy J, Winkler, Kristy R, Mhlanga, Musa, Mizrahi, Valerie, Warner, Digby F
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2020
Materias:
Genetics and Genomics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7647400/
https://www.ncbi.nlm.nih.gov/pubmed/33155979
http://dx.doi.org/10.7554/eLife.60083

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7647400/
https://www.ncbi.nlm.nih.gov/pubmed/33155979
http://dx.doi.org/10.7554/eLife.60083

Ejemplares similares