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Injury triggers fascia fibroblast collective cell migration to drive scar formation through N-cadherin
Scars are more severe when the subcutaneous fascia beneath the dermis is injured upon surgical or traumatic wounding. Here, we present a detailed analysis of fascia cell mobilisation by using deep tissue intravital live imaging of acute surgical wounds, fibroblast lineage-specific transgenic mice, a...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7648088/ https://www.ncbi.nlm.nih.gov/pubmed/33159076 http://dx.doi.org/10.1038/s41467-020-19425-1 |
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author | Jiang, Dongsheng Christ, Simon Correa-Gallegos, Donovan Ramesh, Pushkar Kalgudde Gopal, Shruthi Wannemacher, Juliane Mayr, Christoph H. Lupperger, Valerio Yu, Qing Ye, Haifeng Mück-Häusl, Martin Rajendran, Vijayanand Wan, Li Liu, Juan Mirastschijski, Ursula Volz, Thomas Marr, Carsten Schiller, Herbert B. Rinkevich, Yuval |
author_facet | Jiang, Dongsheng Christ, Simon Correa-Gallegos, Donovan Ramesh, Pushkar Kalgudde Gopal, Shruthi Wannemacher, Juliane Mayr, Christoph H. Lupperger, Valerio Yu, Qing Ye, Haifeng Mück-Häusl, Martin Rajendran, Vijayanand Wan, Li Liu, Juan Mirastschijski, Ursula Volz, Thomas Marr, Carsten Schiller, Herbert B. Rinkevich, Yuval |
author_sort | Jiang, Dongsheng |
collection | PubMed |
description | Scars are more severe when the subcutaneous fascia beneath the dermis is injured upon surgical or traumatic wounding. Here, we present a detailed analysis of fascia cell mobilisation by using deep tissue intravital live imaging of acute surgical wounds, fibroblast lineage-specific transgenic mice, and skin-fascia explants (scar-like tissue in a dish – SCAD). We observe that injury triggers a swarming-like collective cell migration of fascia fibroblasts that progressively contracts the skin and form scars. Swarming is exclusive to fascia fibroblasts, and requires the upregulation of N-cadherin. Both swarming and N-cadherin expression are absent from fibroblasts in the upper skin layers and the oral mucosa, tissues that repair wounds with minimal scar. Impeding N-cadherin binding inhibits swarming and skin contraction, and leads to reduced scarring in SCADs and in animals. Fibroblast swarming and N-cadherin thus provide therapeutic avenues to curtail fascia mobilisation and pathological fibrotic responses across a range of medical settings. |
format | Online Article Text |
id | pubmed-7648088 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-76480882020-11-10 Injury triggers fascia fibroblast collective cell migration to drive scar formation through N-cadherin Jiang, Dongsheng Christ, Simon Correa-Gallegos, Donovan Ramesh, Pushkar Kalgudde Gopal, Shruthi Wannemacher, Juliane Mayr, Christoph H. Lupperger, Valerio Yu, Qing Ye, Haifeng Mück-Häusl, Martin Rajendran, Vijayanand Wan, Li Liu, Juan Mirastschijski, Ursula Volz, Thomas Marr, Carsten Schiller, Herbert B. Rinkevich, Yuval Nat Commun Article Scars are more severe when the subcutaneous fascia beneath the dermis is injured upon surgical or traumatic wounding. Here, we present a detailed analysis of fascia cell mobilisation by using deep tissue intravital live imaging of acute surgical wounds, fibroblast lineage-specific transgenic mice, and skin-fascia explants (scar-like tissue in a dish – SCAD). We observe that injury triggers a swarming-like collective cell migration of fascia fibroblasts that progressively contracts the skin and form scars. Swarming is exclusive to fascia fibroblasts, and requires the upregulation of N-cadherin. Both swarming and N-cadherin expression are absent from fibroblasts in the upper skin layers and the oral mucosa, tissues that repair wounds with minimal scar. Impeding N-cadherin binding inhibits swarming and skin contraction, and leads to reduced scarring in SCADs and in animals. Fibroblast swarming and N-cadherin thus provide therapeutic avenues to curtail fascia mobilisation and pathological fibrotic responses across a range of medical settings. Nature Publishing Group UK 2020-11-06 /pmc/articles/PMC7648088/ /pubmed/33159076 http://dx.doi.org/10.1038/s41467-020-19425-1 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Jiang, Dongsheng Christ, Simon Correa-Gallegos, Donovan Ramesh, Pushkar Kalgudde Gopal, Shruthi Wannemacher, Juliane Mayr, Christoph H. Lupperger, Valerio Yu, Qing Ye, Haifeng Mück-Häusl, Martin Rajendran, Vijayanand Wan, Li Liu, Juan Mirastschijski, Ursula Volz, Thomas Marr, Carsten Schiller, Herbert B. Rinkevich, Yuval Injury triggers fascia fibroblast collective cell migration to drive scar formation through N-cadherin |
title | Injury triggers fascia fibroblast collective cell migration to drive scar formation through N-cadherin |
title_full | Injury triggers fascia fibroblast collective cell migration to drive scar formation through N-cadherin |
title_fullStr | Injury triggers fascia fibroblast collective cell migration to drive scar formation through N-cadherin |
title_full_unstemmed | Injury triggers fascia fibroblast collective cell migration to drive scar formation through N-cadherin |
title_short | Injury triggers fascia fibroblast collective cell migration to drive scar formation through N-cadherin |
title_sort | injury triggers fascia fibroblast collective cell migration to drive scar formation through n-cadherin |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7648088/ https://www.ncbi.nlm.nih.gov/pubmed/33159076 http://dx.doi.org/10.1038/s41467-020-19425-1 |
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