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Optimising total RNA quality and quantity by phenol-chloroform extraction method from human visceral adipose tissue: A standardisation study

A multitude of challenges is faced during RNA extraction from human visceral adipose tissue (VAT) due to its atypical nature and a dearth of existing literature. Our study provides a convenient and inexpensive manual method using TRIzol reagent for the reproducible recovery of intact RNA from sparse...

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Detalles Bibliográficos
Autores principales: Roy, Dipayan, Tomo, Sojit, Modi, Anupama, Purohit, Purvi, Sharma, Praveen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7653057/
https://www.ncbi.nlm.nih.gov/pubmed/33204654
http://dx.doi.org/10.1016/j.mex.2020.101113