LncRNA NEAT1 remodels chromatin to promote the 5-Fu resistance by maintaining colorectal cancer stemness

Resistance of chemotherapy is one of causes of recurrence and poor prognosis in patients with colorectal cancer (CRC). The role of differentially expressed long non-coding RNA (lncRNA) in 5-fluorouracil (5-Fu) resistance has not been fully elucidated. Here we observed that lncRNA NEAT1 was associate...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhu, Yihao, Hu, Hanqing, Yuan, Ziming, Zhang, Qian, Xiong, Huan, Hu, Zhiqiao, Wu, Hongyu, Huang, Rui, Wang, Guiyu, Tang, Qingchao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7653927/
https://www.ncbi.nlm.nih.gov/pubmed/33168814
http://dx.doi.org/10.1038/s41419-020-03164-8
_version_ 1783607974463275008
author Zhu, Yihao
Hu, Hanqing
Yuan, Ziming
Zhang, Qian
Xiong, Huan
Hu, Zhiqiao
Wu, Hongyu
Huang, Rui
Wang, Guiyu
Tang, Qingchao
author_facet Zhu, Yihao
Hu, Hanqing
Yuan, Ziming
Zhang, Qian
Xiong, Huan
Hu, Zhiqiao
Wu, Hongyu
Huang, Rui
Wang, Guiyu
Tang, Qingchao
author_sort Zhu, Yihao
collection PubMed
description Resistance of chemotherapy is one of causes of recurrence and poor prognosis in patients with colorectal cancer (CRC). The role of differentially expressed long non-coding RNA (lncRNA) in 5-fluorouracil (5-Fu) resistance has not been fully elucidated. Here we observed that lncRNA NEAT1 was associated with 5-Fu resistance in CRC. Our Functional studies showed that NEAT1 promoted 5-Fu resistance in colorectal cells. In addition, A-TAC sequencing and chromatin immunoprecipitation (ChIP) showed that NEAT1 affected chromatin remodeling, increased the acetylation levels of histones, increased their enrichment at the promoters of ALDH1 and c-Myc, and promoted the expression of ALDH1 and c-Myc. Taken together, our study suggested that NEAT1 promoted 5-Fu resistance and cancer stemness by remodeling chromatin. Our finding provides a novel role of NEAT1 and may provide a new strategy for the treatment of CRC 5-Fu resistance.
format Online
Article
Text
id pubmed-7653927
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-76539272020-11-12 LncRNA NEAT1 remodels chromatin to promote the 5-Fu resistance by maintaining colorectal cancer stemness Zhu, Yihao Hu, Hanqing Yuan, Ziming Zhang, Qian Xiong, Huan Hu, Zhiqiao Wu, Hongyu Huang, Rui Wang, Guiyu Tang, Qingchao Cell Death Dis Article Resistance of chemotherapy is one of causes of recurrence and poor prognosis in patients with colorectal cancer (CRC). The role of differentially expressed long non-coding RNA (lncRNA) in 5-fluorouracil (5-Fu) resistance has not been fully elucidated. Here we observed that lncRNA NEAT1 was associated with 5-Fu resistance in CRC. Our Functional studies showed that NEAT1 promoted 5-Fu resistance in colorectal cells. In addition, A-TAC sequencing and chromatin immunoprecipitation (ChIP) showed that NEAT1 affected chromatin remodeling, increased the acetylation levels of histones, increased their enrichment at the promoters of ALDH1 and c-Myc, and promoted the expression of ALDH1 and c-Myc. Taken together, our study suggested that NEAT1 promoted 5-Fu resistance and cancer stemness by remodeling chromatin. Our finding provides a novel role of NEAT1 and may provide a new strategy for the treatment of CRC 5-Fu resistance. Nature Publishing Group UK 2020-11-09 /pmc/articles/PMC7653927/ /pubmed/33168814 http://dx.doi.org/10.1038/s41419-020-03164-8 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zhu, Yihao
Hu, Hanqing
Yuan, Ziming
Zhang, Qian
Xiong, Huan
Hu, Zhiqiao
Wu, Hongyu
Huang, Rui
Wang, Guiyu
Tang, Qingchao
LncRNA NEAT1 remodels chromatin to promote the 5-Fu resistance by maintaining colorectal cancer stemness
title LncRNA NEAT1 remodels chromatin to promote the 5-Fu resistance by maintaining colorectal cancer stemness
title_full LncRNA NEAT1 remodels chromatin to promote the 5-Fu resistance by maintaining colorectal cancer stemness
title_fullStr LncRNA NEAT1 remodels chromatin to promote the 5-Fu resistance by maintaining colorectal cancer stemness
title_full_unstemmed LncRNA NEAT1 remodels chromatin to promote the 5-Fu resistance by maintaining colorectal cancer stemness
title_short LncRNA NEAT1 remodels chromatin to promote the 5-Fu resistance by maintaining colorectal cancer stemness
title_sort lncrna neat1 remodels chromatin to promote the 5-fu resistance by maintaining colorectal cancer stemness
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7653927/
https://www.ncbi.nlm.nih.gov/pubmed/33168814
http://dx.doi.org/10.1038/s41419-020-03164-8
work_keys_str_mv AT zhuyihao lncrnaneat1remodelschromatintopromotethe5furesistancebymaintainingcolorectalcancerstemness
AT huhanqing lncrnaneat1remodelschromatintopromotethe5furesistancebymaintainingcolorectalcancerstemness
AT yuanziming lncrnaneat1remodelschromatintopromotethe5furesistancebymaintainingcolorectalcancerstemness
AT zhangqian lncrnaneat1remodelschromatintopromotethe5furesistancebymaintainingcolorectalcancerstemness
AT xionghuan lncrnaneat1remodelschromatintopromotethe5furesistancebymaintainingcolorectalcancerstemness
AT huzhiqiao lncrnaneat1remodelschromatintopromotethe5furesistancebymaintainingcolorectalcancerstemness
AT wuhongyu lncrnaneat1remodelschromatintopromotethe5furesistancebymaintainingcolorectalcancerstemness
AT huangrui lncrnaneat1remodelschromatintopromotethe5furesistancebymaintainingcolorectalcancerstemness
AT wangguiyu lncrnaneat1remodelschromatintopromotethe5furesistancebymaintainingcolorectalcancerstemness
AT tangqingchao lncrnaneat1remodelschromatintopromotethe5furesistancebymaintainingcolorectalcancerstemness

Ejemplares similares