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Green fluorescent protein-based lactate and pyruvate indicators suitable for biochemical assays and live cell imaging
Glycolysis is the metabolic pathway that converts glucose into pyruvate, whereas fermentation can then produce lactate from pyruvate. Here, we developed single fluorescent protein (FP)-based lactate and pyruvate indicators with low EC(50) for trace detection of metabolic molecules and live cell imag...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7659002/ https://www.ncbi.nlm.nih.gov/pubmed/33177605 http://dx.doi.org/10.1038/s41598-020-76440-4 |