Green fluorescent protein-based lactate and pyruvate indicators suitable for biochemical assays and live cell imaging

Glycolysis is the metabolic pathway that converts glucose into pyruvate, whereas fermentation can then produce lactate from pyruvate. Here, we developed single fluorescent protein (FP)-based lactate and pyruvate indicators with low EC(50) for trace detection of metabolic molecules and live cell imag...

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Detalles Bibliográficos
Autores principales: Harada, Kazuki, Chihara, Takami, Hayasaka, Yuki, Mita, Marie, Takizawa, Mai, Ishida, Kentaro, Arai, Mary, Tsuno, Saki, Matsumoto, Mitsuharu, Ishihara, Takeshi, Ueda, Hiroshi, Kitaguchi, Tetsuya, Tsuboi, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7659002/
https://www.ncbi.nlm.nih.gov/pubmed/33177605
http://dx.doi.org/10.1038/s41598-020-76440-4

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