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In Vitro Prebiotic Effects of Malto-Oligosaccharides Containing Water-Soluble Dietary Fiber

This study measured the proliferative activity of malto-oligosaccharide (MOS) as a prebiotic against Bifidobacteria, resistance to digestion in vitro, and changes during in vitro fermentation by human fecal microorganisms. It consisted of 21.74%, 18.84%, and 11.76% of maltotriose, maltotetraose, and...

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Autores principales: Jang, Eun Yeong, Hong, Ki-Bae, Chang, Yeok Boo, Shin, Jungcheul, Jung, Eun Young, Jo, Kyungae, Suh, Hyung Joo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7664926/
https://www.ncbi.nlm.nih.gov/pubmed/33182247
http://dx.doi.org/10.3390/molecules25215201
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author Jang, Eun Yeong
Hong, Ki-Bae
Chang, Yeok Boo
Shin, Jungcheul
Jung, Eun Young
Jo, Kyungae
Suh, Hyung Joo
author_facet Jang, Eun Yeong
Hong, Ki-Bae
Chang, Yeok Boo
Shin, Jungcheul
Jung, Eun Young
Jo, Kyungae
Suh, Hyung Joo
author_sort Jang, Eun Yeong
collection PubMed
description This study measured the proliferative activity of malto-oligosaccharide (MOS) as a prebiotic against Bifidobacteria, resistance to digestion in vitro, and changes during in vitro fermentation by human fecal microorganisms. It consisted of 21.74%, 18.84%, and 11.76% of maltotriose, maltotetraose, and maltopentaose produced by amylase (HATT), respectively. When 1% of MOS was added to a modified PYF medium as the carbon source, proliferation of Bifidobacterium breve was increased significantly. During the in vitro digestion test, MOS was partially degraded by intestinal enzymes. Fermentation characteristics by human fecal microorganisms were evaluated by adding 1% galacto-oligosaccharide (GOS), as well as 1% and 2% MOS as carbon sources to the basal medium, respectively. In comparison with the addition of 1% of MOS and GOS, the total short chain fatty acid (SCFA) content increased over time when 2% of MOS was added. The species diversity and richness of intestinal microbiota increased significantly with 2% MOS compared to those with 1% GOS. In addition, the 2% addition of MOS reduced intestinal pathobiont microorganisms and increased commensal microorganisms including Bifidobacterium genus. Collectively, MOS produced by amylase increased the SCFA production and enhanced the growth of beneficial bacteria during in vitro fermentation by human fecal microbiota.
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spelling pubmed-76649262020-11-14 In Vitro Prebiotic Effects of Malto-Oligosaccharides Containing Water-Soluble Dietary Fiber Jang, Eun Yeong Hong, Ki-Bae Chang, Yeok Boo Shin, Jungcheul Jung, Eun Young Jo, Kyungae Suh, Hyung Joo Molecules Article This study measured the proliferative activity of malto-oligosaccharide (MOS) as a prebiotic against Bifidobacteria, resistance to digestion in vitro, and changes during in vitro fermentation by human fecal microorganisms. It consisted of 21.74%, 18.84%, and 11.76% of maltotriose, maltotetraose, and maltopentaose produced by amylase (HATT), respectively. When 1% of MOS was added to a modified PYF medium as the carbon source, proliferation of Bifidobacterium breve was increased significantly. During the in vitro digestion test, MOS was partially degraded by intestinal enzymes. Fermentation characteristics by human fecal microorganisms were evaluated by adding 1% galacto-oligosaccharide (GOS), as well as 1% and 2% MOS as carbon sources to the basal medium, respectively. In comparison with the addition of 1% of MOS and GOS, the total short chain fatty acid (SCFA) content increased over time when 2% of MOS was added. The species diversity and richness of intestinal microbiota increased significantly with 2% MOS compared to those with 1% GOS. In addition, the 2% addition of MOS reduced intestinal pathobiont microorganisms and increased commensal microorganisms including Bifidobacterium genus. Collectively, MOS produced by amylase increased the SCFA production and enhanced the growth of beneficial bacteria during in vitro fermentation by human fecal microbiota. MDPI 2020-11-09 /pmc/articles/PMC7664926/ /pubmed/33182247 http://dx.doi.org/10.3390/molecules25215201 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jang, Eun Yeong
Hong, Ki-Bae
Chang, Yeok Boo
Shin, Jungcheul
Jung, Eun Young
Jo, Kyungae
Suh, Hyung Joo
In Vitro Prebiotic Effects of Malto-Oligosaccharides Containing Water-Soluble Dietary Fiber
title In Vitro Prebiotic Effects of Malto-Oligosaccharides Containing Water-Soluble Dietary Fiber
title_full In Vitro Prebiotic Effects of Malto-Oligosaccharides Containing Water-Soluble Dietary Fiber
title_fullStr In Vitro Prebiotic Effects of Malto-Oligosaccharides Containing Water-Soluble Dietary Fiber
title_full_unstemmed In Vitro Prebiotic Effects of Malto-Oligosaccharides Containing Water-Soluble Dietary Fiber
title_short In Vitro Prebiotic Effects of Malto-Oligosaccharides Containing Water-Soluble Dietary Fiber
title_sort in vitro prebiotic effects of malto-oligosaccharides containing water-soluble dietary fiber
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7664926/
https://www.ncbi.nlm.nih.gov/pubmed/33182247
http://dx.doi.org/10.3390/molecules25215201
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