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The Challenge of Distinguishing Cell–Cell Complexes from Singlet Cells in Non‐Imaging Flow Cytometry and Single‐Cell Sorting

Our recent work has highlighted that care needs to be taken when interpreting single cell data originating from flow cytometry acquisition or cell sorting: We found that doublets of T cells bound to other immune cells are often present in the live singlet gate of human peripheral blood samples acqui...

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Detalles Bibliográficos
Autores principales: Burel, Julie G., Pomaznoy, Mikhail, Lindestam Arlehamn, Cecilia S., Seumois, Gregory, Vijayanand, Pandurangan, Sette, Alessandro, Peters, Bjoern
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7666012/
https://www.ncbi.nlm.nih.gov/pubmed/32400942
http://dx.doi.org/10.1002/cyto.a.24027