Single-pass transcription by T7 RNA polymerase

RNA molecules can be conveniently synthesized in vitro by the T7 RNA polymerase (T7 RNAP). In some experiments, such as cotranscriptional biochemical analyses, continuous synthesis of RNA is not desired. Here, we propose a method for a single-pass transcription that yields a single transcript per te...

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Detalles Bibliográficos
Autores principales: Passalacqua, Luiz F.M., Dingilian, Armine I., Lupták, Andrej
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2020
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7668259/
https://www.ncbi.nlm.nih.gov/pubmed/32958559
http://dx.doi.org/10.1261/rna.076778.120

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7668259/
https://www.ncbi.nlm.nih.gov/pubmed/32958559
http://dx.doi.org/10.1261/rna.076778.120

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