Cargando…

Single-pass transcription by T7 RNA polymerase

RNA molecules can be conveniently synthesized in vitro by the T7 RNA polymerase (T7 RNAP). In some experiments, such as cotranscriptional biochemical analyses, continuous synthesis of RNA is not desired. Here, we propose a method for a single-pass transcription that yields a single transcript per te...

Descripción completa

Detalles Bibliográficos
Autores principales:	Passalacqua, Luiz F.M., Dingilian, Armine I., Lupták, Andrej
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Cold Spring Harbor Laboratory Press 2020
Materias:	Method
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7668259/ https://www.ncbi.nlm.nih.gov/pubmed/32958559 http://dx.doi.org/10.1261/rna.076778.120

Ejemplares similares

High-throughput, fluorescent-aptamer-based measurements of steady-state transcription rates for the Mycobacterium tuberculosis RNA polymerase
por: Jensen, Drake, et al.
Publicado: (2023)

Comprehensive determination of transcription start sites derived from all RNA polymerases using ReCappable-seq
por: Yan, Bo, et al.
Publicado: (2022)

Regulation of mRNA translation by a photoriboswitch
por: Rotstan, Kelly A, et al.
Publicado: (2020)

In vitro genetic reconstruction of bacterial transcription initiation by coupled synthesis and detection of RNA polymerase holoenzyme
por: Asahara, Haruichi, et al.
Publicado: (2010)

Fast and accurate single-cell RNA-seq analysis by clustering of transcript-compatibility counts
por: Ntranos, Vasilis, et al.
Publicado: (2016)

Affinity purification of T7 RNA transcripts with homogeneous ends using ARiBo and CRISPR tags
por: Salvail-Lacoste, Alix, et al.
Publicado: (2013)

Efficient inhibition of RNA self-primed extension by addition of competing 3′-capture DNA-improved RNA synthesis by T7 RNA polymerase
por: Gholamalipour, Yasaman, et al.
Publicado: (2019)

LAST-seq: single-cell RNA sequencing by direct amplification of single-stranded RNA without prior reverse transcription and second-strand synthesis
por: Lyu, Jun, et al.
Publicado: (2023)

Mapping DNA polymerase errors by single-molecule sequencing
por: Lee, David F., et al.
Publicado: (2016)

Targeted single-cell RNA sequencing of transcription factors enhances the identification of cell types and trajectories
por: Pokhilko, Alexandra, et al.
Publicado: (2021)

An engineered mammalian band-pass network
por: Greber, David, et al.
Publicado: (2010)

Transcript assembly improves expression quantification of transposable elements in single-cell RNA-seq data
por: Shao, Wanqing, et al.
Publicado: (2021)

Synthesis of 2′-Fluoro RNA by Syn5 RNA polymerase
por: Zhu, Bin, et al.
Publicado: (2015)

Linking transcriptional and genetic tumor heterogeneity through allele analysis of single-cell RNA-seq data
por: Fan, Jean, et al.
Publicado: (2018)

Improved genome-wide localization by ChIP-chip using double-round T7 RNA polymerase-based amplification
por: van Bakel, Harm, et al.
Publicado: (2008)

Sierra: discovery of differential transcript usage from polyA-captured single-cell RNA-seq data
por: Patrick, Ralph, et al.
Publicado: (2020)

MAST: a flexible statistical framework for assessing transcriptional changes and characterizing heterogeneity in single-cell RNA sequencing data
por: Finak, Greg, et al.
Publicado: (2015)

Identification of bona fide B2 SINE retrotransposon transcription through single-nucleus RNA-seq of the mouse hippocampus
por: Linker, Sara B., et al.
Publicado: (2020)

Effects of transcriptional noise on estimates of gene and transcript expression in RNA sequencing experiments
por: Varabyou, Ales, et al.
Publicado: (2021)

Short-hairpin RNAs synthesized by T7 phage polymerase do not induce interferon
por: Gondai, Takuma, et al.
Publicado: (2008)

Analysis of RNA Polymerase II Chromatin Binding by Flow Cytometry
por: Bay, Lilli T. E., et al.
Publicado: (2023)

Development of a species-specific RNA polymerase I-based shRNA expression vector
por: Verca, M. S. Brenz, et al.
Publicado: (2007)

Single-cell characterization of CRISPR-modified transcript isoforms with nanopore sequencing
por: Kim, Heon Seok, et al.
Publicado: (2021)

Clinical utilisation of a rapid low-pass whole genome sequencing technique for the diagnosis of aneuploidy in human embryos prior to implantation
por: Wells, Dagan, et al.
Publicado: (2014)

Exploiting single-molecule transcript sequencing for eukaryotic gene prediction
por: Minoche, André E., et al.
Publicado: (2015)

Short amplicon reverse transcription‐polymerase chain reaction detects aberrant splicing in genes with low expression in blood missed by ribonucleic acid sequencing analysis for clinical diagnosis
por: Wai, Htoo A., et al.
Publicado: (2022)

A targeted sequencing extension for transcript genotyping in single-cell transcriptomics
por: Van Horebeek, Lies, et al.
Publicado: (2023)

SplintQuant: a method for accurately quantifying circular RNA transcript abundance without reverse transcription bias
por: Conn, Vanessa, et al.
Publicado: (2019)

Transcriptional bypass of regioisomeric ethylated thymidine lesions by T7 RNA polymerase and human RNA polymerase II
por: You, Changjun, et al.
Publicado: (2014)

Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155
por: Chung, Kwan-Ho, et al.
Publicado: (2006)

By-passing in vitro screening—next generation sequencing technologies applied to antibody display and in silico candidate selection
por: Ravn, U., et al.
Publicado: (2010)

Decontamination of ambient RNA in single-cell RNA-seq with DecontX
por: Yang, Shiyi, et al.
Publicado: (2020)

A split intein T7 RNA polymerase for transcriptional AND-logic
por: Schaerli, Yolanda, et al.
Publicado: (2014)

Engineering efficient termination of bacteriophage T7 RNA polymerase transcription
por: Calvopina-Chavez, Diana G, et al.
Publicado: (2022)

Reverse Transcription Errors and RNA–DNA Differences at Short Tandem Repeats
por: Fungtammasan, Arkarachai, et al.
Publicado: (2016)

Synergising single-cell resolution and 4sU labelling boosts inference of transcriptional bursting
por: Edwards, David M., et al.
Publicado: (2023)

Control of small inhibitory RNA levels and RNA interference by doxycycline induced activation of a minimal RNA polymerase III promoter
por: Amar, Lahouari, et al.
Publicado: (2006)

Integrated detection of natural antisense transcripts using strand-specific RNA sequencing data
por: Li, Song, et al.
Publicado: (2013)

Optimization of oligonucleotide arrays and RNA amplification protocols for analysis of transcript structure and alternative splicing
por: Castle, John, et al.
Publicado: (2003)

Simultaneous detection of mRNA transcription and decay intermediates by dual colour single mRNA FISH on subcellular resolution
por: Kramer, Susanne
Publicado: (2017)

Cannot write session to /tmp/vufind_sessions/sess_djsms2ifprgro1so5npkolbgi1