A versatile toolkit for CRISPR-Cas13-based RNA manipulation in Drosophila

Advances in CRISPR technology have immensely improved our ability to manipulate nucleic acids, and the recent discovery of the RNA-targeting endonuclease Cas13 adds even further functionality. Here, we show that Cas13 works efficiently in Drosophila, both ex vivo and in vivo. We test 44 different Ca...

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Detalles Bibliográficos
Autores principales: Huynh, Nhan, Depner, Noah, Larson, Raegan, King-Jones, Kirst
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7670108/
https://www.ncbi.nlm.nih.gov/pubmed/33203452
http://dx.doi.org/10.1186/s13059-020-02193-y

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7670108/
https://www.ncbi.nlm.nih.gov/pubmed/33203452
http://dx.doi.org/10.1186/s13059-020-02193-y

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