Protocatechuic aldehyde from Salvia miltiorrhiza exhibits an anti-inflammatory effect through inhibiting MAPK signalling pathway

BACKGROUND: The aerial parts of Salvia miltiorrhiza, which was considered to be the waste part and discarded during the root harvest, is rich in protocatechuic aldehyde (PAI). This study investigated the health-promoting effects of extracts and PAI from the aerial parts of Salvia miltiorrhiza, inclu...

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Autores principales: Wu, Shuang, Wang, Qingyu, Wang, Jinquan, Duan, Baoyu, Tang, Qihe, Sun, Zhuojian, Han, Jinlong, Shan, Chenggang, Wang, Zhifen, Hao, Zhihui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7670718/
https://www.ncbi.nlm.nih.gov/pubmed/33203388
http://dx.doi.org/10.1186/s12906-020-03090-4
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author Wu, Shuang
Wang, Qingyu
Wang, Jinquan
Duan, Baoyu
Tang, Qihe
Sun, Zhuojian
Han, Jinlong
Shan, Chenggang
Wang, Zhifen
Hao, Zhihui
author_facet Wu, Shuang
Wang, Qingyu
Wang, Jinquan
Duan, Baoyu
Tang, Qihe
Sun, Zhuojian
Han, Jinlong
Shan, Chenggang
Wang, Zhifen
Hao, Zhihui
author_sort Wu, Shuang
collection PubMed
description BACKGROUND: The aerial parts of Salvia miltiorrhiza, which was considered to be the waste part and discarded during the root harvest, is rich in protocatechuic aldehyde (PAI). This study investigated the health-promoting effects of extracts and PAI from the aerial parts of Salvia miltiorrhiza, including its anti-inflammatory effects and the underlying mechanisms of action in vitro and in vivo. METHOD: Purification of the sample paste of Salvia miltiorrhiza was accomplished using HPLC analysis. TheMTT (Methylthiazolyldiphenyl-tetrazolium bromide) assay was employed to determine the cell viability. The production of inflammatory factors was detected by ELISA assays. The histopathological analysis was used to analyse the lungs and livers of mice treated with PAI. Western blot was performed to reveal the mechanism of PAI in anti-inflammatory. RESULTS: The extracts and PAI from the aerial parts of Salvia miltiorrhiza inhibited TNF-α, IL-6 production and promoted the production of IL-10 in vivo in mice and in vitro in the macrophage cell line RAW264.7. NF-κB and MAPKs kinase phosphorylation were also suppressed by PAI in vivo and in vitro, indicating that PAI exhibited an anti-inflammatory effect. CONCLUSION: These findings suggest that the aerial parts of Salvia miltiorrhiza extract may serve as potential protective agents for inflammatory. SUPPLEMENTARY INFORMATION: Supplementary information accompanies this paper at 10.1186/s12906-020-03090-4.
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spelling pubmed-76707182020-11-18 Protocatechuic aldehyde from Salvia miltiorrhiza exhibits an anti-inflammatory effect through inhibiting MAPK signalling pathway Wu, Shuang Wang, Qingyu Wang, Jinquan Duan, Baoyu Tang, Qihe Sun, Zhuojian Han, Jinlong Shan, Chenggang Wang, Zhifen Hao, Zhihui BMC Complement Med Ther Research Article BACKGROUND: The aerial parts of Salvia miltiorrhiza, which was considered to be the waste part and discarded during the root harvest, is rich in protocatechuic aldehyde (PAI). This study investigated the health-promoting effects of extracts and PAI from the aerial parts of Salvia miltiorrhiza, including its anti-inflammatory effects and the underlying mechanisms of action in vitro and in vivo. METHOD: Purification of the sample paste of Salvia miltiorrhiza was accomplished using HPLC analysis. TheMTT (Methylthiazolyldiphenyl-tetrazolium bromide) assay was employed to determine the cell viability. The production of inflammatory factors was detected by ELISA assays. The histopathological analysis was used to analyse the lungs and livers of mice treated with PAI. Western blot was performed to reveal the mechanism of PAI in anti-inflammatory. RESULTS: The extracts and PAI from the aerial parts of Salvia miltiorrhiza inhibited TNF-α, IL-6 production and promoted the production of IL-10 in vivo in mice and in vitro in the macrophage cell line RAW264.7. NF-κB and MAPKs kinase phosphorylation were also suppressed by PAI in vivo and in vitro, indicating that PAI exhibited an anti-inflammatory effect. CONCLUSION: These findings suggest that the aerial parts of Salvia miltiorrhiza extract may serve as potential protective agents for inflammatory. SUPPLEMENTARY INFORMATION: Supplementary information accompanies this paper at 10.1186/s12906-020-03090-4. BioMed Central 2020-11-17 /pmc/articles/PMC7670718/ /pubmed/33203388 http://dx.doi.org/10.1186/s12906-020-03090-4 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Wu, Shuang
Wang, Qingyu
Wang, Jinquan
Duan, Baoyu
Tang, Qihe
Sun, Zhuojian
Han, Jinlong
Shan, Chenggang
Wang, Zhifen
Hao, Zhihui
Protocatechuic aldehyde from Salvia miltiorrhiza exhibits an anti-inflammatory effect through inhibiting MAPK signalling pathway
title Protocatechuic aldehyde from Salvia miltiorrhiza exhibits an anti-inflammatory effect through inhibiting MAPK signalling pathway
title_full Protocatechuic aldehyde from Salvia miltiorrhiza exhibits an anti-inflammatory effect through inhibiting MAPK signalling pathway
title_fullStr Protocatechuic aldehyde from Salvia miltiorrhiza exhibits an anti-inflammatory effect through inhibiting MAPK signalling pathway
title_full_unstemmed Protocatechuic aldehyde from Salvia miltiorrhiza exhibits an anti-inflammatory effect through inhibiting MAPK signalling pathway
title_short Protocatechuic aldehyde from Salvia miltiorrhiza exhibits an anti-inflammatory effect through inhibiting MAPK signalling pathway
title_sort protocatechuic aldehyde from salvia miltiorrhiza exhibits an anti-inflammatory effect through inhibiting mapk signalling pathway
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7670718/
https://www.ncbi.nlm.nih.gov/pubmed/33203388
http://dx.doi.org/10.1186/s12906-020-03090-4
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