Substrate specificity of 2-deoxy-D-ribose 5-phosphate aldolase (DERA) assessed by different protein engineering and machine learning methods

ABSTRACT: In this work, deoxyribose-5-phosphate aldolase (Ec DERA, EC 4.1.2.4) from Escherichia coli was chosen as the protein engineering target for improving the substrate preference towards smaller, non-phosphorylated aldehyde donor substrates, in particular towards acetaldehyde. The initial broa...

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Detalles Bibliográficos
Autores principales: Voutilainen, Sanni, Heinonen, Markus, Andberg, Martina, Jokinen, Emmi, Maaheimo, Hannu, Pääkkönen, Johan, Hakulinen, Nina, Rouvinen, Juha, Lähdesmäki, Harri, Kaski, Samuel, Rousu, Juho, Penttilä, Merja, Koivula, Anu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Biotechnologically Relevant Enzymes and Proteins
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7671976/
https://www.ncbi.nlm.nih.gov/pubmed/33147349
http://dx.doi.org/10.1007/s00253-020-10960-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7671976/
https://www.ncbi.nlm.nih.gov/pubmed/33147349
http://dx.doi.org/10.1007/s00253-020-10960-x

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