Protein identification by 3D OrbiSIMS to facilitate in situ imaging and depth profiling

Label-free protein characterization at surfaces is commonly achieved using digestion and/or matrix application prior to mass spectrometry. We report the assignment of undigested proteins at surfaces in situ using secondary ion mass spectrometry (SIMS). Ballistic fragmentation of proteins induced by...

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Detalles Bibliográficos
Autores principales: Kotowska, Anna M., Trindade, Gustavo F., Mendes, Paula M., Williams, Philip M., Aylott, Jonathan W., Shard, Alexander G., Alexander, Morgan R., Scurr, David J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7672064/
https://www.ncbi.nlm.nih.gov/pubmed/33203841
http://dx.doi.org/10.1038/s41467-020-19445-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7672064/
https://www.ncbi.nlm.nih.gov/pubmed/33203841
http://dx.doi.org/10.1038/s41467-020-19445-x

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