Systematic use of synthetic 5′-UTR RNA structures to tune protein translation improves yield and quality of complex proteins in mammalian cell factories

Predictably regulating protein expression levels to improve recombinant protein production has become an important tool, but is still rarely applied to engineer mammalian cells. We therefore sought to set-up an easy-to-implement toolbox to facilitate fast and reliable regulation of protein expressio...

Descripción completa

Detalles Bibliográficos
Autores principales: Eisenhut, Peter, Mebrahtu, Aman, Moradi Barzadd, Mona, Thalén, Niklas, Klanert, Gerald, Weinguny, Marcus, Sandegren, Anna, Su, Chao, Hatton, Diane, Borth, Nicole, Rockberg, Johan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7672427/
https://www.ncbi.nlm.nih.gov/pubmed/33051690
http://dx.doi.org/10.1093/nar/gkaa847

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7672427/
https://www.ncbi.nlm.nih.gov/pubmed/33051690
http://dx.doi.org/10.1093/nar/gkaa847

Ejemplares similares