Physiological Traits of Dihomo-γ-Linolenic Acid Production of the Engineered Aspergillus oryzae by Comparing Mathematical Models
Dihomo-γ-linolenic acid (DGLA; C20:3 n-6) is expected to dominate the functional ingredients market for its role in anti-inflammation and anti-proliferation. The DGLA production by the engineered strain of Aspergillus oryzae with overexpressing Pythium Δ(6)-desaturase and Δ(6)-elongase genes was inv...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7674286/ https://www.ncbi.nlm.nih.gov/pubmed/33224108 http://dx.doi.org/10.3389/fmicb.2020.546230 |
_version_ | 1783611471921414144 |
---|---|
author | Antimanon, Sompot Anantayanon, Jutamas Wannawilai, Siwaporn Khongto, Bhimabol Laoteng, Kobkul |
author_facet | Antimanon, Sompot Anantayanon, Jutamas Wannawilai, Siwaporn Khongto, Bhimabol Laoteng, Kobkul |
author_sort | Antimanon, Sompot |
collection | PubMed |
description | Dihomo-γ-linolenic acid (DGLA; C20:3 n-6) is expected to dominate the functional ingredients market for its role in anti-inflammation and anti-proliferation. The DGLA production by the engineered strain of Aspergillus oryzae with overexpressing Pythium Δ(6)-desaturase and Δ(6)-elongase genes was investigated by manipulating the nutrient and fermentation regimes. Of the nitrogen sources tested, the maximum biomass and DGLA titers were obtained in the cultures using NaNO(3) grown at pH 6.0. For establishing economically feasible process of DGLA production, the cost-effective medium was developed by using cassava starch hydrolysate (CSH) and NaNO(3) as carbon and nitrogen sources, respectively. The supplementation with 1% (v/v) mother liquor (ML) into the CSH medium promoted the specific yield of DGLA production (Y(DGLA)(/)(X)) comparable with the culture grown in the defined NaNO(3) medium, and the DGLA proportion was over 22% in total fatty acid (TFA). Besides, the GLA was also generated at a similar proportion (about 25% in TFA). The mathematical models of the cultures grown in the defined NaNO(3) and CSH/ML media were generated, describing that the lipid and DGLA were growth-associated metabolites corresponding to the relevant kinetic parameters of fermentations. The controlled mode of submerged fermentation of the engineered strain was explored for governing the PUFA biosynthesis and lipid-accumulating process in relation to the biomass production. This study provides an informative perspective in the n-6 fatty acid production through physiological manipulation, thus leading to a prospect in viable production of the DGLA-enriched oil by the engineered strain. |
format | Online Article Text |
id | pubmed-7674286 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-76742862020-11-19 Physiological Traits of Dihomo-γ-Linolenic Acid Production of the Engineered Aspergillus oryzae by Comparing Mathematical Models Antimanon, Sompot Anantayanon, Jutamas Wannawilai, Siwaporn Khongto, Bhimabol Laoteng, Kobkul Front Microbiol Microbiology Dihomo-γ-linolenic acid (DGLA; C20:3 n-6) is expected to dominate the functional ingredients market for its role in anti-inflammation and anti-proliferation. The DGLA production by the engineered strain of Aspergillus oryzae with overexpressing Pythium Δ(6)-desaturase and Δ(6)-elongase genes was investigated by manipulating the nutrient and fermentation regimes. Of the nitrogen sources tested, the maximum biomass and DGLA titers were obtained in the cultures using NaNO(3) grown at pH 6.0. For establishing economically feasible process of DGLA production, the cost-effective medium was developed by using cassava starch hydrolysate (CSH) and NaNO(3) as carbon and nitrogen sources, respectively. The supplementation with 1% (v/v) mother liquor (ML) into the CSH medium promoted the specific yield of DGLA production (Y(DGLA)(/)(X)) comparable with the culture grown in the defined NaNO(3) medium, and the DGLA proportion was over 22% in total fatty acid (TFA). Besides, the GLA was also generated at a similar proportion (about 25% in TFA). The mathematical models of the cultures grown in the defined NaNO(3) and CSH/ML media were generated, describing that the lipid and DGLA were growth-associated metabolites corresponding to the relevant kinetic parameters of fermentations. The controlled mode of submerged fermentation of the engineered strain was explored for governing the PUFA biosynthesis and lipid-accumulating process in relation to the biomass production. This study provides an informative perspective in the n-6 fatty acid production through physiological manipulation, thus leading to a prospect in viable production of the DGLA-enriched oil by the engineered strain. Frontiers Media S.A. 2020-11-05 /pmc/articles/PMC7674286/ /pubmed/33224108 http://dx.doi.org/10.3389/fmicb.2020.546230 Text en Copyright © 2020 Antimanon, Anantayanon, Wannawilai, Khongto and Laoteng. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Antimanon, Sompot Anantayanon, Jutamas Wannawilai, Siwaporn Khongto, Bhimabol Laoteng, Kobkul Physiological Traits of Dihomo-γ-Linolenic Acid Production of the Engineered Aspergillus oryzae by Comparing Mathematical Models |
title | Physiological Traits of Dihomo-γ-Linolenic Acid Production of the Engineered Aspergillus oryzae by Comparing Mathematical Models |
title_full | Physiological Traits of Dihomo-γ-Linolenic Acid Production of the Engineered Aspergillus oryzae by Comparing Mathematical Models |
title_fullStr | Physiological Traits of Dihomo-γ-Linolenic Acid Production of the Engineered Aspergillus oryzae by Comparing Mathematical Models |
title_full_unstemmed | Physiological Traits of Dihomo-γ-Linolenic Acid Production of the Engineered Aspergillus oryzae by Comparing Mathematical Models |
title_short | Physiological Traits of Dihomo-γ-Linolenic Acid Production of the Engineered Aspergillus oryzae by Comparing Mathematical Models |
title_sort | physiological traits of dihomo-γ-linolenic acid production of the engineered aspergillus oryzae by comparing mathematical models |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7674286/ https://www.ncbi.nlm.nih.gov/pubmed/33224108 http://dx.doi.org/10.3389/fmicb.2020.546230 |
work_keys_str_mv | AT antimanonsompot physiologicaltraitsofdihomoglinolenicacidproductionoftheengineeredaspergillusoryzaebycomparingmathematicalmodels AT anantayanonjutamas physiologicaltraitsofdihomoglinolenicacidproductionoftheengineeredaspergillusoryzaebycomparingmathematicalmodels AT wannawilaisiwaporn physiologicaltraitsofdihomoglinolenicacidproductionoftheengineeredaspergillusoryzaebycomparingmathematicalmodels AT khongtobhimabol physiologicaltraitsofdihomoglinolenicacidproductionoftheengineeredaspergillusoryzaebycomparingmathematicalmodels AT laotengkobkul physiologicaltraitsofdihomoglinolenicacidproductionoftheengineeredaspergillusoryzaebycomparingmathematicalmodels |