Bicuspid valve aortopathy is associated with distinct patterns of matrix degradation

OBJECTIVE: To explore the micromechanical, biochemical, and microstructural differences between bicuspid aortic valve aneurysm (BAV-A) and tricuspid aortic valve idiopathic degenerative aneurysm (DA), compared with normal aorta. METHODS: Aortic tissue was obtained from patients undergoing aneurysmal...

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Autores principales: Chim, Ya Hua, Davies, Hannah A., Mason, David, Nawaytou, Omar, Field, Mark, Madine, Jillian, Akhtar, Riaz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mosby 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7674632/
https://www.ncbi.nlm.nih.gov/pubmed/31679706
http://dx.doi.org/10.1016/j.jtcvs.2019.08.094
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author Chim, Ya Hua
Davies, Hannah A.
Mason, David
Nawaytou, Omar
Field, Mark
Madine, Jillian
Akhtar, Riaz
author_facet Chim, Ya Hua
Davies, Hannah A.
Mason, David
Nawaytou, Omar
Field, Mark
Madine, Jillian
Akhtar, Riaz
author_sort Chim, Ya Hua
collection PubMed
description OBJECTIVE: To explore the micromechanical, biochemical, and microstructural differences between bicuspid aortic valve aneurysm (BAV-A) and tricuspid aortic valve idiopathic degenerative aneurysm (DA), compared with normal aorta. METHODS: Aortic tissue was obtained from patients undergoing aneurysmal repair surgery (BAV-A; n = 15 and DA; n = 15). Control tissue was obtained from aortic punch biopsies during coronary artery bypass graft surgery (n = 9). Nanoindentation was used to determine the elastic modulus on the medial layer. Glycosaminoglycan, collagen, and elastin levels were measured using biochemical assays. Verhoeff Van Gieson–stained cross-sections were imaged for elastin microstructural quantification. RESULTS: The elastic modulus was more than 20% greater for BAV-A relative to control and DA (signifying a loss of compliance). No significance difference between control and DA were observed. Collagen levels for BAV-A (36.9 ± 7.4 μg/mg) and DA (49.9 ± 10.9 μg/mg) were greater compared with the control (30.2 ± 13.1 μg/mg). Glycosaminoglycan and elastin levels were not significant between the groups. Elastin segments were uniform throughout the control. Aneurysmal tissues had less elastin segments close to the intima and adventitia layers. Both BAV-A and DA had elastin segments compacted in the media; however, elastin segments were highly fragmented in DA. CONCLUSIONS: BAV-A has a greater loss of aortic wall compliance relative to DA and the control. Although elastin levels were equal for all groups, spatial distribution of elastin provided a unique profile of matrix degradation for BAV-A. Elastin compaction within the media of BAV-A may have resulted from the altered hemodynamic pressure against the wall, which could explain for the stiffness of the tissue.
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spelling pubmed-76746322020-12-01 Bicuspid valve aortopathy is associated with distinct patterns of matrix degradation Chim, Ya Hua Davies, Hannah A. Mason, David Nawaytou, Omar Field, Mark Madine, Jillian Akhtar, Riaz J Thorac Cardiovasc Surg Adult: Aortic Valve: Basic Science OBJECTIVE: To explore the micromechanical, biochemical, and microstructural differences between bicuspid aortic valve aneurysm (BAV-A) and tricuspid aortic valve idiopathic degenerative aneurysm (DA), compared with normal aorta. METHODS: Aortic tissue was obtained from patients undergoing aneurysmal repair surgery (BAV-A; n = 15 and DA; n = 15). Control tissue was obtained from aortic punch biopsies during coronary artery bypass graft surgery (n = 9). Nanoindentation was used to determine the elastic modulus on the medial layer. Glycosaminoglycan, collagen, and elastin levels were measured using biochemical assays. Verhoeff Van Gieson–stained cross-sections were imaged for elastin microstructural quantification. RESULTS: The elastic modulus was more than 20% greater for BAV-A relative to control and DA (signifying a loss of compliance). No significance difference between control and DA were observed. Collagen levels for BAV-A (36.9 ± 7.4 μg/mg) and DA (49.9 ± 10.9 μg/mg) were greater compared with the control (30.2 ± 13.1 μg/mg). Glycosaminoglycan and elastin levels were not significant between the groups. Elastin segments were uniform throughout the control. Aneurysmal tissues had less elastin segments close to the intima and adventitia layers. Both BAV-A and DA had elastin segments compacted in the media; however, elastin segments were highly fragmented in DA. CONCLUSIONS: BAV-A has a greater loss of aortic wall compliance relative to DA and the control. Although elastin levels were equal for all groups, spatial distribution of elastin provided a unique profile of matrix degradation for BAV-A. Elastin compaction within the media of BAV-A may have resulted from the altered hemodynamic pressure against the wall, which could explain for the stiffness of the tissue. Mosby 2020-12 /pmc/articles/PMC7674632/ /pubmed/31679706 http://dx.doi.org/10.1016/j.jtcvs.2019.08.094 Text en © 2019 by The American Association for Thoracic Surgery. Published by Elsevier Inc. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Adult: Aortic Valve: Basic Science
Chim, Ya Hua
Davies, Hannah A.
Mason, David
Nawaytou, Omar
Field, Mark
Madine, Jillian
Akhtar, Riaz
Bicuspid valve aortopathy is associated with distinct patterns of matrix degradation
title Bicuspid valve aortopathy is associated with distinct patterns of matrix degradation
title_full Bicuspid valve aortopathy is associated with distinct patterns of matrix degradation
title_fullStr Bicuspid valve aortopathy is associated with distinct patterns of matrix degradation
title_full_unstemmed Bicuspid valve aortopathy is associated with distinct patterns of matrix degradation
title_short Bicuspid valve aortopathy is associated with distinct patterns of matrix degradation
title_sort bicuspid valve aortopathy is associated with distinct patterns of matrix degradation
topic Adult: Aortic Valve: Basic Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7674632/
https://www.ncbi.nlm.nih.gov/pubmed/31679706
http://dx.doi.org/10.1016/j.jtcvs.2019.08.094
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