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Cryo-electron microscopy structures of pyrene-labeled ADP-P(i)- and ADP-actin filaments
Since the fluorescent reagent N-(1-pyrene)iodoacetamide was first used to label skeletal muscle actin in 1981, the pyrene-labeled actin has become the most widely employed tool to measure the kinetics of actin polymerization and the interaction between actin and actin-binding proteins. Here we repor...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Nature Publishing Group UK
2020
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7677365/ https://www.ncbi.nlm.nih.gov/pubmed/33214556 http://dx.doi.org/10.1038/s41467-020-19762-1 |