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Single-cell analysis of autophagy activity in normal and de novo transformed human mammary cells

Assessment of autophagy activity has historically been limited to investigations of fixed tissue or bulk cell populations. To address questions of heterogeneity and relate measurements to functional properties of viable cells isolated from primary tissue, we created a lentiviral (RFP-GFP-MAP1LC3B) v...

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Detalles Bibliográficos
Autores principales: Lefort, Sylvain, Balani, Sneha, Pellacani, Davide, Guyot, Boris, Gorski, Sharon M., Maguer-Satta, Véronique, Eaves, Connie J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7679376/
https://www.ncbi.nlm.nih.gov/pubmed/33219251
http://dx.doi.org/10.1038/s41598-020-77347-w