Cargando…
Dinucleosome specificity and allosteric switch of the ISW1a ATP-dependent chromatin remodeler in transcription regulation
Over the last 3 decades ATP-dependent chromatin remodelers have been thought to recognize chromatin at the level of single nucleosomes rather than higher-order organization of more than one nucleosome. We show the yeast ISW1a remodeler has such higher-order structural specificity, as manifested by l...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7680125/ https://www.ncbi.nlm.nih.gov/pubmed/33219211 http://dx.doi.org/10.1038/s41467-020-19700-1 |
_version_ | 1783612402007277568 |
---|---|
author | Bhardwaj, Saurabh K. Hailu, Solomon G. Olufemi, Lola Brahma, Sandipan Kundu, Soumyadipta Hota, Swetansu K. Persinger, Jim Bartholomew, Blaine |
author_facet | Bhardwaj, Saurabh K. Hailu, Solomon G. Olufemi, Lola Brahma, Sandipan Kundu, Soumyadipta Hota, Swetansu K. Persinger, Jim Bartholomew, Blaine |
author_sort | Bhardwaj, Saurabh K. |
collection | PubMed |
description | Over the last 3 decades ATP-dependent chromatin remodelers have been thought to recognize chromatin at the level of single nucleosomes rather than higher-order organization of more than one nucleosome. We show the yeast ISW1a remodeler has such higher-order structural specificity, as manifested by large allosteric changes that activate the nucleosome remodeling and spacing activities of ISW1a when bound to dinucleosomes. Although the ATPase domain of Isw1 docks at the SHL2 position when ISW1a is bound to either mono- or di-nucleosomes, there are major differences in the interactions of the catalytic subunit Isw1 with the acidic pocket of nucleosomes and the accessory subunit Ioc3 with nucleosomal DNA. By mutational analysis and uncoupling of ISW1a’s dinucleosome specificity, we find that dinucleosome recognition is required by ISW1a for proper chromatin organization at promoters; as well as transcription regulation in combination with the histone acetyltransferase NuA4 and histone H2A.Z exchanger SWR1. |
format | Online Article Text |
id | pubmed-7680125 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-76801252020-11-24 Dinucleosome specificity and allosteric switch of the ISW1a ATP-dependent chromatin remodeler in transcription regulation Bhardwaj, Saurabh K. Hailu, Solomon G. Olufemi, Lola Brahma, Sandipan Kundu, Soumyadipta Hota, Swetansu K. Persinger, Jim Bartholomew, Blaine Nat Commun Article Over the last 3 decades ATP-dependent chromatin remodelers have been thought to recognize chromatin at the level of single nucleosomes rather than higher-order organization of more than one nucleosome. We show the yeast ISW1a remodeler has such higher-order structural specificity, as manifested by large allosteric changes that activate the nucleosome remodeling and spacing activities of ISW1a when bound to dinucleosomes. Although the ATPase domain of Isw1 docks at the SHL2 position when ISW1a is bound to either mono- or di-nucleosomes, there are major differences in the interactions of the catalytic subunit Isw1 with the acidic pocket of nucleosomes and the accessory subunit Ioc3 with nucleosomal DNA. By mutational analysis and uncoupling of ISW1a’s dinucleosome specificity, we find that dinucleosome recognition is required by ISW1a for proper chromatin organization at promoters; as well as transcription regulation in combination with the histone acetyltransferase NuA4 and histone H2A.Z exchanger SWR1. Nature Publishing Group UK 2020-11-20 /pmc/articles/PMC7680125/ /pubmed/33219211 http://dx.doi.org/10.1038/s41467-020-19700-1 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Bhardwaj, Saurabh K. Hailu, Solomon G. Olufemi, Lola Brahma, Sandipan Kundu, Soumyadipta Hota, Swetansu K. Persinger, Jim Bartholomew, Blaine Dinucleosome specificity and allosteric switch of the ISW1a ATP-dependent chromatin remodeler in transcription regulation |
title | Dinucleosome specificity and allosteric switch of the ISW1a ATP-dependent chromatin remodeler in transcription regulation |
title_full | Dinucleosome specificity and allosteric switch of the ISW1a ATP-dependent chromatin remodeler in transcription regulation |
title_fullStr | Dinucleosome specificity and allosteric switch of the ISW1a ATP-dependent chromatin remodeler in transcription regulation |
title_full_unstemmed | Dinucleosome specificity and allosteric switch of the ISW1a ATP-dependent chromatin remodeler in transcription regulation |
title_short | Dinucleosome specificity and allosteric switch of the ISW1a ATP-dependent chromatin remodeler in transcription regulation |
title_sort | dinucleosome specificity and allosteric switch of the isw1a atp-dependent chromatin remodeler in transcription regulation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7680125/ https://www.ncbi.nlm.nih.gov/pubmed/33219211 http://dx.doi.org/10.1038/s41467-020-19700-1 |
work_keys_str_mv | AT bhardwajsaurabhk dinucleosomespecificityandallostericswitchoftheisw1aatpdependentchromatinremodelerintranscriptionregulation AT hailusolomong dinucleosomespecificityandallostericswitchoftheisw1aatpdependentchromatinremodelerintranscriptionregulation AT olufemilola dinucleosomespecificityandallostericswitchoftheisw1aatpdependentchromatinremodelerintranscriptionregulation AT brahmasandipan dinucleosomespecificityandallostericswitchoftheisw1aatpdependentchromatinremodelerintranscriptionregulation AT kundusoumyadipta dinucleosomespecificityandallostericswitchoftheisw1aatpdependentchromatinremodelerintranscriptionregulation AT hotaswetansuk dinucleosomespecificityandallostericswitchoftheisw1aatpdependentchromatinremodelerintranscriptionregulation AT persingerjim dinucleosomespecificityandallostericswitchoftheisw1aatpdependentchromatinremodelerintranscriptionregulation AT bartholomewblaine dinucleosomespecificityandallostericswitchoftheisw1aatpdependentchromatinremodelerintranscriptionregulation |