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High CD45 expression of CD8+ and CD4+ T cells correlates with the size of HIV-1 reservoir in blood
Using mass cytometry, we investigated the expression of 28 markers on CD8+ and CD4+ T cells from HIV-1 infected patients with a variable size of HIV-1 reservoir defined as high (HR) and low (LR) reservoir; we aimed at identifying phenotypic associations of T cells with size of HIV-1 reservoir. We sh...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7686502/ https://www.ncbi.nlm.nih.gov/pubmed/33235273 http://dx.doi.org/10.1038/s41598-020-77433-z |
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author | Petkov, Stefan Bekele, Yonas Lakshmikanth, Tadepally Hejdeman, Bo Zazzi, Maurizio Brodin, Petter Chiodi, Francesca |
author_facet | Petkov, Stefan Bekele, Yonas Lakshmikanth, Tadepally Hejdeman, Bo Zazzi, Maurizio Brodin, Petter Chiodi, Francesca |
author_sort | Petkov, Stefan |
collection | PubMed |
description | Using mass cytometry, we investigated the expression of 28 markers on CD8+ and CD4+ T cells from HIV-1 infected patients with a variable size of HIV-1 reservoir defined as high (HR) and low (LR) reservoir; we aimed at identifying phenotypic associations of T cells with size of HIV-1 reservoir. We showed that the frequency of CD45+ CD8+ and CD4+ T cells was directly proportional to the size of HIV-1 reservoir; HR patients had a significantly larger frequency of blood CD45(high) T cells and higher CD45 expression on both CD8+ and CD4+ T cells. CD45 is a receptor-type protein tyrosine phosphatase essential in TCR signaling. Functional and phenotypical analysis of CD45(high) cells revealed that they express activation and proliferation markers (CD38 + HLA-DR + and Ki-67) and produce cytokines upon in vitro activation. CD45(high) T cells also expressed high levels of immune check-point PD-1. Our results link CD45 expression on T cells to HIV-1 reservoir; PD-1 expression on CD45(high) T cells may contribute to their exhaustion. |
format | Online Article Text |
id | pubmed-7686502 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-76865022020-11-27 High CD45 expression of CD8+ and CD4+ T cells correlates with the size of HIV-1 reservoir in blood Petkov, Stefan Bekele, Yonas Lakshmikanth, Tadepally Hejdeman, Bo Zazzi, Maurizio Brodin, Petter Chiodi, Francesca Sci Rep Article Using mass cytometry, we investigated the expression of 28 markers on CD8+ and CD4+ T cells from HIV-1 infected patients with a variable size of HIV-1 reservoir defined as high (HR) and low (LR) reservoir; we aimed at identifying phenotypic associations of T cells with size of HIV-1 reservoir. We showed that the frequency of CD45+ CD8+ and CD4+ T cells was directly proportional to the size of HIV-1 reservoir; HR patients had a significantly larger frequency of blood CD45(high) T cells and higher CD45 expression on both CD8+ and CD4+ T cells. CD45 is a receptor-type protein tyrosine phosphatase essential in TCR signaling. Functional and phenotypical analysis of CD45(high) cells revealed that they express activation and proliferation markers (CD38 + HLA-DR + and Ki-67) and produce cytokines upon in vitro activation. CD45(high) T cells also expressed high levels of immune check-point PD-1. Our results link CD45 expression on T cells to HIV-1 reservoir; PD-1 expression on CD45(high) T cells may contribute to their exhaustion. Nature Publishing Group UK 2020-11-24 /pmc/articles/PMC7686502/ /pubmed/33235273 http://dx.doi.org/10.1038/s41598-020-77433-z Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Petkov, Stefan Bekele, Yonas Lakshmikanth, Tadepally Hejdeman, Bo Zazzi, Maurizio Brodin, Petter Chiodi, Francesca High CD45 expression of CD8+ and CD4+ T cells correlates with the size of HIV-1 reservoir in blood |
title | High CD45 expression of CD8+ and CD4+ T cells correlates with the size of HIV-1 reservoir in blood |
title_full | High CD45 expression of CD8+ and CD4+ T cells correlates with the size of HIV-1 reservoir in blood |
title_fullStr | High CD45 expression of CD8+ and CD4+ T cells correlates with the size of HIV-1 reservoir in blood |
title_full_unstemmed | High CD45 expression of CD8+ and CD4+ T cells correlates with the size of HIV-1 reservoir in blood |
title_short | High CD45 expression of CD8+ and CD4+ T cells correlates with the size of HIV-1 reservoir in blood |
title_sort | high cd45 expression of cd8+ and cd4+ t cells correlates with the size of hiv-1 reservoir in blood |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7686502/ https://www.ncbi.nlm.nih.gov/pubmed/33235273 http://dx.doi.org/10.1038/s41598-020-77433-z |
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