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Fluorescent multiplexing of 3D spheroids: Analysis of biomarkers using automated immunohistochemistry staining platform and multispectral imaging

In preclinical cancer studies, three‐dimensional (3D) cell spheroids and aggregates are preferred over monolayer cell cultures due to their architectural and functional similarity to solid tumors. We performed a proof‐of‐concept study to generate physiologically relevant and predictive preclinical m...

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Detalles Bibliográficos
Autores principales: Bhaumik, Srabani, Boyer, Jean, Banerjee, Chaitali, Clark, Samantha, Sebastiao, Noemi, Vela, Elizabeth, Towne, Penny
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7689845/
https://www.ncbi.nlm.nih.gov/pubmed/32692912
http://dx.doi.org/10.1002/jcb.29827